Laboratory of Cell Recognition and Pattern Formation, Graduate School of Biostudies, Kyoto University, Sakyo-ku, Kyoto 606-8501, Japan.
Laboratory of Theoretical Biology, Graduate School of Biostudies, Kyoto University, Sakyo-ku, Kyoto 606-8501, Japan; Faculty of Medicine, Kyoto University, Sakyo-ku, Kyoto 606-8501, Japan.
Cell Rep. 2023 Jul 25;42(7):112707. doi: 10.1016/j.celrep.2023.112707. Epub 2023 Jul 10.
During development, positional information directs cells to specific fates, leading them to differentiate with their own transcriptomes and express specific behaviors and functions. However, the mechanisms underlying these processes in a genome-wide view remain ambiguous, partly because the single-cell transcriptomic data of early developing embryos containing accurate spatial and lineage information are still lacking. Here, we report a single-cell transcriptome atlas of Drosophila gastrulae, divided into 77 transcriptomically distinct clusters. We find that the expression profiles of plasma-membrane-related genes, but not those of transcription-factor genes, represent each germ layer, supporting the nonequivalent contribution of each transcription-factor mRNA level to effector gene expression profiles at the transcriptome level. We also reconstruct the spatial expression patterns of all genes at the single-cell stripe level as the smallest unit. This atlas is an important resource for the genome-wide understanding of the mechanisms by which genes cooperatively orchestrate Drosophila gastrulation.
在发育过程中，位置信息指导细胞走向特定的命运，使它们通过自己的转录组分化，并表现出特定的行为和功能。然而，由于缺乏包含准确空间和谱系信息的早期胚胎的单细胞转录组数据，这些过程在全基因组范围内的机制仍不清楚。在这里，我们报告了果蝇原肠胚的单细胞转录组图谱，该图谱分为 77 个转录上不同的簇。我们发现，与转录因子基因不同，与质膜相关的基因的表达谱代表了每个胚层，这支持了每个转录因子 mRNA 水平在转录组水平上对效应基因表达谱的非等效贡献。我们还在单细胞条带水平上重建了所有基因的空间表达模式，这是最小的单位。这个图谱是全面了解基因协同调控果蝇原肠胚形成的机制的重要资源。
