Department of Engineering, University of Campania "L. Vanvitelli", Via Roma 29, Aversa, Italy.
CNRS, IS2M UMR 7361, University of Upper-Alsace, 68100, Mulhouse, France.
Sci Rep. 2023 Jul 11;13(1):11210. doi: 10.1038/s41598-023-38262-y.
The simultaneous interrogation of both lossy mode (LMR) and surface plasmon (SPR) resonances was herein exploited for the first time to devise a sensor in combination with soft molecularly imprinting of nanoparticles (nanoMIPs), specifically entailed of the selectivity towards the protein biomarker human serum transferrin (HTR). Two distinct metal-oxide bilayers, i.e. TiO-ZrO and ZrO-TiO, were used in the SPR-LMR sensing platforms. The responses to binding of the target protein HTR of both sensing configurations (TiO-ZrO-Au-nanoMIPs, ZrO-TiO-Au-nanoMIPs) showed femtomolar HTR detection, LODs of tens of fM and K ~ 30 fM. Selectivity for HTR was demonstrated. The SPR interrogation was more efficient for the ZrO-TiO-Au-nanoMIPs configuration (sensitivity at low concentrations, S = 0.108 nm/fM) than for the TiO-ZrO-Au-nanoMIPs one (S = 0.061 nm/fM); while LMR was more efficient for TiO-ZrO-Au-nanoMIPs (S = 0.396 nm/fM) than for ZrO-TiO-Au-nanoMIPs (S = 0.177 nm/fM). The simultaneous resonance monitoring is advantageous for point of care determinations, both in terms of measurement's redundancy, that enables the cross-control of the measure and the optimization of the detection, by exploiting the individual characteristics of each resonance.
本文首次利用同时检测损耗模式 (LMR) 和表面等离子体 (SPR) 共振的方法，设计了一种与纳米粒子软分子印迹 (nanoMIPs) 相结合的传感器，特别是对蛋白质生物标志物人血清转铁蛋白 (HTR) 具有选择性。两种不同的金属氧化物双层，即 TiO-ZrO 和 ZrO-TiO，被用于 SPR-LMR 传感平台。两种传感配置（TiO-ZrO-Au-nanoMIPs、ZrO-TiO-Au-nanoMIPs）对靶蛋白 HTR 的结合响应均表现出皮摩尔级的 HTR 检测、数十 fM 的 LOD 和 K ~ 30 fM。证明了对 HTR 的选择性。SPR 检测对于 ZrO-TiO-Au-nanoMIPs 配置（在低浓度下的灵敏度，S = 0.108 nm/fM）比 TiO-ZrO-Au-nanoMIPs 配置（S = 0.061 nm/fM）更有效；而 LMR 对于 TiO-ZrO-Au-nanoMIPs（S = 0.396 nm/fM）比 ZrO-TiO-Au-nanoMIPs（S = 0.177 nm/fM）更有效。同时监测共振对于即时护理测定是有利的，这在测量的冗余性方面，通过利用每个共振的特性，可以交叉控制测量并优化检测。
