综合分析以确定LINC00511-hsa-miR-625-5p-SEMA6A通路促进皮肤黑色素瘤进展
Comprehensive Analysis to Identify LINC00511-hsa-miR-625-5p-SEMA6A Pathway Fuels Progression of Skin Cutaneous Melanoma.
作者信息
Chen Guanghua, Yan Jia, Fu Zhou
机构信息
Department of Dermatology, Children's Hospital of Chongqing Medical University, National Clinical Research Center for Child Health and Disorders, Ministry of Education Key Laboratory of Child Development and Disorders, Chongqing Key Laboratory of Child Infection and Immunity, Chongqing 400014, China.
Department of General Surgery, University-Town Hospital of Chongqing Medical University, Chongqing 401331, China.
出版信息
Int J Genomics. 2023 Jul 3;2023:6422941. doi: 10.1155/2023/6422941. eCollection 2023.
OBJECTIVE
Skin cutaneous melanoma (SKCM) is a highly lethal malignancy that poses a significant threat to human health. Recent research has shown that competing endogenous RNA (ceRNA) regulatory networks play a critical role in the development and progression of various types of cancer, including SKCM. The objective of this study is to investigate the ceRNA regulatory network associated with the transmembrane protein semaphorin 6A (SEMA6A) and identify the underlying molecular mechanisms involved in SKCM.
METHODS
Expression profiles of four RNAs, including pseudogenes, long non-coding RNAs, microRNAs, and mRNAs were obtained from The Cancer Genome Atlas database. The analysis was completed by bioinformatics methods, and the expression levels of the selected genes were verified by cell experiments.
RESULTS
Bioinformatics analysis revealed that the LINC00511-hsa-miR-625-5p-SEMA6A ceRNA network was associated with SKCM prognosis. Furthermore, immune infiltration analysis indicated that the LINC00511-hsa-miR-625-5p-SEMA6A axis may have an impact on changes in the tumor immune microenvironment of SKCM.
CONCLUSION
The LINC00511-hsa-miR-625-5p-SEMA6A axis could be a promising therapeutic target and a prognostic biomarker for SKCM.
目的
皮肤黑色素瘤（SKCM）是一种高度致命的恶性肿瘤，对人类健康构成重大威胁。最近的研究表明，竞争性内源性RNA（ceRNA）调控网络在包括SKCM在内的各种癌症的发生和发展中起着关键作用。本研究的目的是探讨与跨膜蛋白信号素6A（SEMA6A）相关的ceRNA调控网络，并确定SKCM中潜在的分子机制。
方法
从癌症基因组图谱数据库中获取包括假基因、长链非编码RNA、微小RNA和信使RNA在内的四种RNA的表达谱。通过生物信息学方法完成分析，并通过细胞实验验证所选基因的表达水平。
结果
生物信息学分析显示，LINC00511-hsa-miR-625-5p-SEMA6A ceRNA网络与SKCM预后相关。此外，免疫浸润分析表明，LINC00511-hsa-miR-625-5p-SEMA6A轴可能对SKCM肿瘤免疫微环境的变化产生影响。
结论
LINC00511-hsa-miR-625-5p-SEMA6A轴可能是SKCM一个有前景的治疗靶点和预后生物标志物。
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