大肠杆菌中环丙烷脂肪酸的性质与生物合成
Properties and biosynthesis of cyclopropane fatty acids in Escherichia coli.
作者信息
Cronan J E, Reed R, Taylor F R, Jackson M B
出版信息
J Bacteriol. 1979 Apr;138(1):118-21. doi: 10.1128/jb.138.1.118-121.1979.
Abstract
The lipid phase transition of Escherichia coli phospholipids containing cyclopropane fatty acids was compared with the otherwise homologous phospholipids lacking cyclopropane fatty acids. The phase transitions (determined by scanning calorimetry) of the two preparations were essentially identical. Infection of E. coli with phage T3 inhibited cyclopropane fatty acid formation over 98%, whereas infection with mutants which lack the phage coded S-adenosylmethionine cleavage enzyme had no effect on cyclopropane fatty acid synthesis. These data indicate that S-adenosylmethionine is the methylene in cyclopropane fatty acid synthesis.
摘要
将含有环丙烷脂肪酸的大肠杆菌磷脂的脂相转变与不含环丙烷脂肪酸的其他同源磷脂进行了比较。两种制剂的相转变（通过扫描量热法测定）基本相同。用噬菌体T3感染大肠杆菌可抑制98%以上的环丙烷脂肪酸形成，而用缺乏噬菌体编码的S-腺苷甲硫氨酸裂解酶的突变体感染对环丙烷脂肪酸合成没有影响。这些数据表明，S-腺苷甲硫氨酸是环丙烷脂肪酸合成中的亚甲基。
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本文引用的文献
1
S-ADENOSYLMETHIONINE IN THE BIOSYNTHESIS OF BACTERIAL FATTY ACIDS.细菌脂肪酸生物合成中的S-腺苷甲硫氨酸
J Bacteriol. 1962 Nov;84(5):967-72. doi: 10.1128/jb.84.5.967-972.1962.
2
Utilization of S-adenosylmethionine by micro-organisms.微生物对S-腺苷甲硫氨酸的利用
J Bacteriol. 1962 Jan;83(1):169-74. doi: 10.1128/jb.83.1.169-174.1962.
3
Acetylornithinase of Escherichia coli: partial purification and some properties.大肠杆菌的乙酰鸟氨酸酶：部分纯化及某些性质
J Biol Chem. 1956 Jan;218(1):97-106.
4
5
The effects of temperature on the composition and physical properties of the lipids of Pseudomonas fluorescens.温度对荧光假单胞菌脂质组成和物理性质的影响。
Biochem J. 1971 Dec;125(3):733-42. doi: 10.1042/bj1250733.
6
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Proc Natl Acad Sci U S A. 1970 Oct;67(2):606-12. doi: 10.1073/pnas.67.2.606.
7
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J Virol. 1967 Feb;1(1):57-63. doi: 10.1128/jvi.1.1.57-63.1967.
8
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9
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Adv Microb Physiol. 1972;8:1-58. doi: 10.1016/s0065-2911(08)60187-3.
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