Overath P, Schairer H U, Stoffel W
Proc Natl Acad Sci U S A. 1970 Oct;67(2):606-12. doi: 10.1073/pnas.67.2.606.
A double mutant of Escherichia coli unable to synthesize or degrade unsaturated fatty acids can incorporate fatty acids with various hydrocarbon chain structures into the membrane phospholipids. The temperature characteristic of three physiological properties of cells grown with different fatty acids (growth, respiration, and efflux of thiomethylgalactoside) is compared with the physical properties of the isolated phosphatidylethanolamines in monolayers at an air-water interface. Breaks in the temperature characteristic of the properties measured in vivo correspond to phase transitions in the lipid films from a liquid-expanded to a condensed form. It is concluded that a liquid-like state of the lipid phase is required for proper membrane function.
一种无法合成或降解不饱和脂肪酸的大肠杆菌双突变体能够将具有各种烃链结构的脂肪酸掺入膜磷脂中。将用不同脂肪酸培养的细胞的三种生理特性(生长、呼吸和硫代甲基半乳糖苷流出)的温度特性与在空气-水界面单层中分离的磷脂酰乙醇胺的物理特性进行比较。体内测量特性的温度特性中的断点对应于脂质膜从液体膨胀形式到凝聚形式的相变。得出的结论是,脂质相的类液态状态是膜正常功能所必需的。