Croute F, Vidal S, Soleilhavoup J P, Vincent C, Serre G, Planel H
Exp Gerontol. 1986;21(1):1-11. doi: 10.1016/0531-5565(86)90012-4.
Among the various parameters that are supposed to play a role in aging at the cellular level, the "free radical theory" involves biochemical modifications that can be induced by radiation. Human embryonic lung fibroblasts were serially subcultivated at low density under chronic low dose rate irradiation (40 mrad/day) and in a normal environment. Irradiation increases cell attachment and the population doubling/day throughout their entire in vitro lifespan. Consequently, the doubling potential reached by irradiated cells was higher than that of control cultures. Finally, the total number of cells produced under chronic irradiation was 8-14 times higher than in a normal environment. These results are discussed with respect to the increased enzymatic activities (superoxidismutase, catalase, glutathion-reductase, G6PD) found in some irradiated organisms.
在细胞水平上被认为在衰老过程中起作用的各种参数中，“自由基理论”涉及可由辐射诱导的生化修饰。人胚肺成纤维细胞在慢性低剂量率照射（40毫拉德/天）下和正常环境中以低密度连续传代培养。在其整个体外寿命期间，照射增加了细胞贴壁和每天的群体倍增数。因此，照射细胞达到的倍增潜能高于对照培养物。最后，慢性照射下产生的细胞总数比正常环境中高8至14倍。结合在一些受照射生物体中发现的酶活性（超氧化物歧化酶、过氧化氢酶、谷胱甘肽还原酶、葡萄糖-6-磷酸脱氢酶）增加对这些结果进行了讨论。
