Sowers A E, Lieber M R
FEBS Lett. 1986 Sep 15;205(2):179-84. doi: 10.1016/0014-5793(86)80893-6.
Low light level video microscopy was used to study the diameter, lifetime, number, and location characteristics of electric field-induced pores (electropores) in erythrocyte ghosts. The diameter of electropores was probed by following the efflux of soluble fluorescent-tagged molecules out of the resealed ghost cytoplasmic compartments. After reaching a peak radius of at least 8.4 nm the electropores resealed within 200 ms to a radius of about 0.5 nm and stayed at that radius thereafter. Video sequences clearly show that pores are induced preferentially in the cathodal hemisphere. Pores induced in the hemisphere facing the positive electrode were either never greater than 0.5 nm in radius, much smaller in number if they were greater than 0.5 nm in radius, or shorter lived. Calculations indicated that an upper limit of 700 electropores were induced per membrane.
采用弱光水平视频显微镜研究了红细胞血影中电场诱导孔（电穿孔）的直径、寿命、数量和位置特征。通过追踪可溶性荧光标记分子从重新封闭的血影细胞质区室中流出，探测电穿孔的直径。在达到至少8.4nm的峰值半径后，电穿孔在200ms内重新封闭至约0.5nm的半径，并在此后保持该半径。视频序列清楚地表明，孔优先在阴极半球诱导形成。在面向正电极的半球中诱导形成的孔，其半径从未大于0.5nm，如果半径大于0.5nm，则数量少得多，或者寿命更短。计算表明，每个膜诱导形成的电穿孔上限为700个。
