聚乙二醇对裂殖酵母电穿孔孔大小的影响。
Influence of Polyethylene Glycol on the Size of Schizosaccharomyces pombe Electropores.
机构信息
Department of Biology, Boston College, Chestnut Hill, Massachusetts 02167-3811.
出版信息
Appl Environ Microbiol. 1992 Apr;58(4):1201-6. doi: 10.1128/aem.58.4.1201-1206.1992.
The role of polyethylene glycol (PEG) in the transformation of Schizosaccharomyces pombe by electroporation is investigated by fluorescein isothiocyanate-dextran uptake and transformation studies. It is shown that when S. pombe cells are electroporated in the presence of PEG, the permeability state created is sustained until removal of PEG. In addition, the permeability of electroporated S. pombe envelopes is further increased with longer incubation times in PEG. The increased permeability is apparently a result of enlarged pores (electropores) due to the presence of PEG. Comparison of a heat pulse transformation protocol with electroporation suggests a second role for PEG in the uptake of macromolecules. Since pores are not thought to be created during a heat pulse, the PEG may be facilitating the uptake of plasmid DNA. This facilitation of uptake would also be expected to affect DNA uptake by electroporated cells.
聚乙二醇（PEG）在电穿孔转化酿酒酵母中的作用通过荧光素异硫氰酸酯-葡聚糖摄取和转化研究进行了研究。结果表明，当酿酒酵母细胞在 PEG 的存在下电穿孔时，创建的通透性状态持续存在，直到 PEG 被去除。此外，在 PEG 中孵育更长时间会进一步增加电穿孔酿酒酵母囊泡的通透性。这种增加的通透性显然是由于 PEG 的存在而导致的孔（电穿孔）扩大的结果。与电穿孔相比，热脉冲转化方案的比较表明 PEG 在大分子摄取中具有第二个作用。由于在热脉冲过程中不认为会产生孔，因此 PEG 可能有助于质粒 DNA 的摄取。这种摄取的促进作用也预计会影响电穿孔细胞的 DNA 摄取。
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