Department of Clinical Laboratory Sciences, College of Applied Medical Sciences, King Saud University, Riyadh 11433, Saudi Arabia.
Molecules. 2022 Nov 2;27(21):7463. doi: 10.3390/molecules27217463.
Propolis, popularly known as bee glue, is a resinous, sticky substance produced by different bee species across the globe. Studies on the biological properties of propolis from the Philippines are rare. Hence, the current study aims at the chemical characterization of propolis produced by the stingless bees Friese from the Philippines and to investigate its antitrypanosomal and anticancer properties. The determination of the chemical composition and characterization of propolis samples was achieved using liquid chromatography-mass spectrometry (LC-MS), -high-performance liquid chromatography-evaporative light scattering detector (HPLC-ELSD), and nuclear magnetic resonance (NMR) spectroscopy. Three major triterpenes were isolated and identified using HRESI-MS and H/C NMR techniques. The spectral studies confirmed the presence of compounds such as isomangiferolic acid, 27-hydoxymangiferonic acid, and 27-hydroxyisomangiferolic acid. All crude propolis samples, isolated fractions, and pure compounds demonstrated moderate antitrypanosomal and anticancer properties compared to control drugs. Amongst the tested compounds, 27-hydoxymangiferonic acid exhibited the highest antitrypanosomal activity at a concentration of 11.6 µg/mL. The highest anticancer effect was demonstrated by the Ph-2 fraction, followed by 27-hydroxyisomangiferolic acid, with IC50 values of 129.6 and 153.3 µg/mL. Thus, it can be concluded that the observed biological activity of Philippine propolis is due to the combinatorial effect or synergistic action of the active compounds 27-hydoxymangiferonic acid and 27-hydroxyisomangiferolic acid.
蜂胶，俗称蜜蜂胶，是全球不同蜜蜂物种产生的一种树脂状、粘性物质。关于菲律宾蜂胶的生物特性研究很少。因此，本研究旨在对菲律宾无刺蜜蜂 Friese 所产蜂胶进行化学特征分析，并研究其抗锥虫和抗癌特性。采用液相色谱-质谱联用（LC-MS）、高效液相色谱-蒸发光散射检测器（HPLC-ELSD）和核磁共振（NMR）光谱法对蜂胶样品的化学成分和特性进行了测定。采用 HRESI-MS 和 H/C NMR 技术分离和鉴定了三种主要的三萜类化合物。光谱研究证实了存在异芒果酸、27-羟芒果酸和 27-羟基异芒果酸等化合物。与对照药物相比，所有粗蜂胶样品、分离馏分和纯化合物均表现出中等的抗锥虫和抗癌特性。在所测试的化合物中，27-羟芒果酸在 11.6µg/mL 的浓度下表现出最高的抗锥虫活性。Ph-2 馏分表现出最高的抗癌活性，其次是 27-羟基异芒果酸，IC50 值分别为 129.6µg/mL 和 153.3µg/mL。因此，可以得出结论，菲律宾蜂胶的观察到的生物活性是由于活性化合物 27-羟芒果酸和 27-羟基异芒果酸的组合效应或协同作用所致。
