Center of Biophotonics, School of Dentistry, Federal University of Bahia, 62, Araujo Pinho Ave, Canela, Salvador, BA, CEP 40110-150, Brazil.
Center for Orthodontics and Facial Orthopedics Prof. José Édimo Soares Martins, School of Dentistry, Federal University of Bahia, 62, Araujo Pinho Ave, Canela, Salvador, BA, CEP 40110-150, Brazil.
Lasers Med Sci. 2023 Jul 13;38(1):159. doi: 10.1007/s10103-023-03825-y.
The aim of this study was to evaluate the influence of IR (λ850 ± 10 nm) and violet (λ405 ± 10 nm) LED phototherapy on total mast cells counts and its ability to influence mast cell degranulation. For this, 27 Wistar rats were used and were randomly distributed into three groups: control, IR LED, and violet LED. When indicated, irradiation done and they were sacrificed, had their tongue removed immediately, 20-min, 45-min, and 2-h after irradiation. Samples were processed to wax, cut, and stained with Toluidine Blue. Intact and degranulated mast cells were counted under light microscopy, and statistical analysis was carried out. In the superficial connective tissue and muscular tissues, violet LED light caused a significant increase in both total number and degranulated mast cells when compared to the control group immediately after irradiation. The degranulation indexes were higher in the groups irradiated with Violet light, both in superficial connective tissue and muscular tissues in relation to the timing. Irradiation with IR LED caused immediate increase in the total number and degranulated of mast cells when compared to the control group only in the superficial connective tissue. In all times observed, the highest total amount of mast cells was seen immediately after irradiation, except in the muscular tissue, which presented the highest amount after 20-min. It was concluded that IR and violet LED light were able to increase the number of mast cells and inducing degranulation in oral mucosa. However, considering that violet LED light can be harmful in periodontal disease, it seems that the use of IR LED light could be the best option in Dentistry.
本研究旨在评估 IR（λ850±10nm）和紫光（λ405±10nm）LED 光疗对总肥大细胞计数的影响及其对肥大细胞脱颗粒的影响。为此，使用了 27 只 Wistar 大鼠，并将其随机分为三组：对照组、IR LED 组和紫光 LED 组。当照射时，大鼠被处死，立即从舌部取出样本，在照射后 20min、45min 和 2h 进行分析。样本经过石蜡包埋、切片和甲苯胺蓝染色。在光镜下对完整和脱颗粒的肥大细胞进行计数，并进行统计学分析。在浅层结缔组织和肌肉组织中，与对照组相比，紫光 LED 光照射后即刻总肥大细胞数和脱颗粒肥大细胞数均显著增加。与光照时间相比，紫光灯照射组的脱颗粒指数在浅层结缔组织和肌肉组织中均较高。与对照组相比，IR LED 照射仅在浅层结缔组织中即刻增加了总肥大细胞数和脱颗粒肥大细胞数。在所有观察时间点，除肌肉组织外，照射后即刻的肥大细胞总量最高，肌肉组织在 20min 后达到最高水平。结论：IR 和紫光 LED 光均可增加口腔黏膜肥大细胞数量并诱导脱颗粒。然而，鉴于紫光 LED 光在牙周病中可能有害，IR LED 光的应用似乎是牙科的最佳选择。
