Li Lixuan, Chen Xiaochun, Zhang Keying, Tian Gang, Ding Xuemei, Bai Shiping, Zeng Qiufeng
Key Laboratory of Animal Disease-Resistance Nutrition, Ministry of Education, Ministry of Agriculture and Rural Affairs, Key Laboratory of Sichuan Province, Animal Nutrition Institute, Sichuan Agricultural University, Chengdu 611130, China.
Institute of Animal Science, Chengdu Agricultural College, Chengdu 611130, China.
Animals (Basel). 2023 Jul 7;13(13):2242. doi: 10.3390/ani13132242.
This study aimed to evaluate the effect of diets supplementing with various levels of thymol and carvacrol eutectic (TCE) on growth performance, serum biochemical parameters, intestinal morphology, and the expression of intestinal nutrient absorption, barrier function- and inflammation-related genes in broiler chickens. A total of 640 one-day-old Arbor Acres male broilers with similar body weights were randomly divided into four groups (8 replicates/group, 20 broilers/replicate). Birds in the four experimental groups were fed a basal diet with TCE at 0, 30, 60, or 120 mg/kg. The results showed that the growth performance of birds during 22-42 d or 1-42 d, serum IgE and IgG content at 21 d of age, jejunal and ileal morphology, ileal , , and mRNA expression were significantly increased compared with the control group ( < 0.05), and the ileal mRNA expression quadratically decreased ( < 0.05) with increasing dietary TCE supplemented dosage, and its expression showed a linear downward trend (0.05 < < 0.1). Meanwhile, compared with the other three groups, birds fed diets with 30 mg/kg TCE presented better ( < 0.05) growth performance, intestinal morphology, and function. These results indicated that the optimal supplementation amount of TCE in the broiler diets was 30 mg/kg.
本研究旨在评估添加不同水平百里香酚和香芹酚共晶物（TCE）的日粮对肉鸡生长性能、血清生化参数、肠道形态以及肠道营养吸收、屏障功能和炎症相关基因表达的影响。选取640只体重相近的1日龄艾维茵雄性肉鸡，随机分为四组（每组8个重复，每个重复20只肉鸡）。四个实验组的肉鸡分别饲喂添加0、30、60或120 mg/kg TCE的基础日粮。结果表明，与对照组相比，22 - 42日龄或1 - 42日龄肉鸡的生长性能、21日龄时血清IgE和IgG含量、空肠和回肠形态、回肠 、 以及 mRNA表达均显著增加（P < 0.05），回肠 mRNA表达随日粮TCE添加量的增加呈二次下降（P < 0.05），且其表达呈线性下降趋势（0.05 < P < 0.1）。同时，与其他三组相比，饲喂30 mg/kg TCE日粮的肉鸡生长性能、肠道形态和功能更佳（P < 0.05）。这些结果表明，肉鸡日粮中TCE的最佳添加量为30 mg/kg。
