Bose S K, Goswami P C
Infect Immun. 1986 Sep;53(3):646-50. doi: 10.1128/iai.53.3.646-650.1986.
Treatment of HeLa 229 cultures with 17-beta-estradiol or with diethylstilbestrol, a synthetic estrogen analog, prior to infection with Chlamydia trachomatis UW31 (serovar K) or LGV440 (serovar L1) led to a 50 to 60% enhancement of chlamydial inclusion formation. After infection, the presence of estrogen was required for the enhancement. The optimal concentration of estrogen required was 10(-10) M. At least 18 h of preinfection treatment plus 12 h of postinfection treatment was necessary. The adherence of purified radioactive elementary bodies of C. trachomatis to estrogen-treated HeLa cells was stimulated in an estrogen dose- and exposure-dependent manner. The requirements for both pre- and postinfection exposure to the hormone suggest that alterations in the cell membrane as well as in the metabolic capacity of the host cells is required for intracellular chlamydial development. Cycloheximide did not prevent estrogen enhancement of chlamydial adherence or subsequent intracellular development of inclusions.
在用沙眼衣原体UW31（血清型K）或LGV440（血清型L1）感染之前，用17-β-雌二醇或合成雌激素类似物己烯雌酚处理HeLa 229培养物，可使衣原体包涵体形成增加50%至60%。感染后，雌激素的存在是增强所必需的。所需雌激素的最佳浓度为10^(-10) M。感染前至少需要18小时的处理加上感染后12小时的处理。沙眼衣原体纯化的放射性原体对雌激素处理的HeLa细胞的粘附以雌激素剂量和暴露依赖性方式受到刺激。感染前和感染后暴露于该激素的要求表明，细胞内衣原体发育需要细胞膜以及宿主细胞代谢能力的改变。放线菌酮并不能阻止雌激素增强衣原体的粘附或随后包涵体的细胞内发育。
