Sugarman B, Epps L R
Infect Immun. 1982 Feb;35(2):633-8. doi: 10.1128/iai.35.2.633-638.1982.
Incubating confluent cell culture HeLa cells for 18 h with increasing concentrations of estrogens progressively enhanced the subsequent attachment of a variety of radiolabeled bacteria to the HeLa cells. This effect was not caused by other hormones and was not produced by 1-h incubations of HeLa cells or bacteria with hormones. Estrogens did not similarly affect two other receptor cell lines studied. The addition of metabolic inhibitors showed that this effect of estrogens on HeLa cells was energy dependent and involved protein synthesis. Concurrent incubation of the HeLa cells and estrogens with the antiestrogen nafoxidine blocked the subsequent increase in adherence. These data suggest that estrogen receptors are present in HeLa cells and that hormonally-induced alterations in the synthesis of bacterial receptor sites may modify the capacity of certain cells to bind bacteria.
用浓度递增的雌激素将汇合的HeLa细胞培养物孵育18小时，会逐渐增强随后多种放射性标记细菌与HeLa细胞的附着。这种效应不是由其他激素引起的，也不是通过HeLa细胞或细菌与激素孵育1小时产生的。雌激素对另外两种研究的受体细胞系没有类似影响。添加代谢抑制剂表明，雌激素对HeLa细胞的这种效应是能量依赖性的，并且涉及蛋白质合成。HeLa细胞、雌激素与抗雌激素萘福昔定同时孵育可阻断随后的黏附增加。这些数据表明HeLa细胞中存在雌激素受体，并且激素诱导的细菌受体位点合成改变可能会改变某些细胞结合细菌的能力。
