Disinfection Supply Center, Lishui Second People's Hospital, Lishui, China.
Department of Stomatology, Beijing Xicheng District Health Care Center for Mothers and Children, Beijing, China.
Front Cell Infect Microbiol. 2022 May 20;12:892049. doi: 10.3389/fcimb.2022.892049. eCollection 2022.
, a golden oyster mushroom, is popular in Asia and has pharmacological functions. However, the effects of polysaccharide-peptides extracted from and underlying mechanism on digestive systme have not yet been clarified. Here, we determined the composition of two polysaccharide-peptides (PSI and PSII) from and investigated the protective effects of on hepatoprotective and gut microbiota. The results showed that PSI and PSII were made up of similar monosaccharide moieties, except for the varying ratios. Furthermore, PSI and PSII showed that they have the hepatoprotective effects and significantly increased the viabilities and cellular total superoxide dismutase activities increased significantly in HepG2 cells. Intracellular triglyceride content and extracellular alanine aminotransferase and aspartate transaminase contents markedly decreased following treatment with 40 and 50 μg/mL PSI and PSII, respectively. Moreover, PSI and PSII activated the adiponectin pathway and reduced lipid accumulation in liver cells. PSI and PSII elevated short-chain fatty acid concentrations, especially butyric and acetic acids. 16S rRNA gene sequencing analysis showed that PSI promoted the relative abundances of , , , as well as generas in the gut. PSII markedly suppressed the relative abundances of and generas. We speculate that the PSI and PSII play a role through liver-gut axis system. Polysaccharide-peptides metabolize by gut microbiota to produce short-chain fatty acids (SCFAs) and in turn influence liver functions.
金耳，一种在亚洲广受欢迎的食用蘑菇，具有药理学功能。然而，金耳中提取的多糖肽的作用及其对消化系统的潜在机制尚未阐明。在这里，我们确定了两种来自金耳的多糖肽（PSI 和 PSII）的组成，并研究了它们对肝脏保护和肠道微生物群的保护作用。结果表明，PSI 和 PSII 由相似的单糖组成，除了比例不同。此外，PSI 和 PSII 表现出对 HepG2 细胞具有肝保护作用，可显著提高细胞活力和细胞总超氧化物歧化酶活性。用 40 和 50μg/ml 的 PSI 和 PSII 处理后，细胞内甘油三酯含量以及细胞外丙氨酸转氨酶和天冬氨酸转氨酶含量显著降低。此外，PSI 和 PSII 激活了脂联素途径，减少了肝细胞中的脂质积累。PSI 和 PSII 提高了短链脂肪酸的浓度，特别是丁酸和乙酸的浓度。16S rRNA 基因测序分析表明，PSI 促进了肠道中 、 、 以及 属的相对丰度增加。PSII 显著抑制了 和 属的相对丰度。我们推测 PSI 和 PSII 通过肝肠轴系统发挥作用。多糖肽通过肠道微生物群代谢产生短链脂肪酸（SCFAs），进而影响肝脏功能。
