Exploration and Evaluation of Secondary Metabolites from : GC-MS Analysis, Phytochemical Profiling, Antifungal and Antioxidant Activity Assessment.

In this study, we investigated in vitro the potential of to produce bioactive secondary metabolites that can be used as alternatives to synthetic compounds. The study focused on analyzing two extracts of using ethyl acetate and -butanol solvents with different polarities. The extracts were examined using phytochemical analysis to determine the content of polyphenols, flavonoids, tannins, and alkaloids. Thin-layer chromatography (TLC) and Gas chromatography-mass spectroscopy (GC-MS) analysis were used to profile volatile organic metabolites (VOCs) present in the extracts. Furthermore, the extracts were tested for their antifungal ability using the poison food technique. For measuring antioxidant activity, the 1,1-diphenyl-2-picryl-hydrazyl (DPPH) test was used. was shown to have a significantly high content of tannins and alkaloids, with a noticeable difference between the two extracts. GC-MS analysis identified 33 potential compounds with numerous benefits that could be used in agriculture and the medicinal industry. Moreover, strong antifungal activity was identified against by 94.44%, sp. by 77.04%, and by 51.48; similarly, the IC of antioxidant activity was estimated for ethyl acetate extract by 71.47% and -butanol extract by 56.01%. This leads to the conclusion that VOCs play a significant role as an antifungal and antioxidant agent when taking into account the advantageous bioactive chemicals noted in the extracts. However, to our knowledge, this is the first study in Algeria presenting detailed phytochemical analysis and GC-MS profiling of for two extracts, ethyl acetate and -butanol.