Laboratory of Interfaces and Advanced Materials, Faculty of Sciences of Monastir, University of Monastir, Monastir 5000, Tunisia.
Laboratory of Bioresources: Integrative Biology and Exploiting, Higher Institute of Biotechnology of Monastir, University of Monastir, Monastir 5000, Tunisia.
Molecules. 2020 Oct 15;25(20):4739. doi: 10.3390/molecules25204739.
The aim of this work was to perform an unprecedented in-depth study on the bioactive phytochemicals of L. Moench Tunisian landrace (Marsaouia). For this purpose, its nutritional, aroma volatile, and phenolic profiles were characterized, and sundry biological activities were assessed The approximate composition revealed that total dietary fiber as the most abundant macronutrient, mainly insoluble dietary fiber, followed by total carbohydrates and proteins. In addition, okra pods were rich in K, Ca, Mg, organic acids, tocopherols, and chlorophylls. Gas Chromatography-Electron Impact Mass Spectrometry (GC-EIMS) analysis showed that oxygenated monoterpenes, sesquiterpene hydrocarbons, and phenylpropanoids were the predominant essential volatile components in pods. A total of eight flavonols were detected by High-Performance Liquid Chromatography coupled to a DAD detector and mass spectrometry by electrospray ionization (HPLC-DAD-MS/ESI); with quercetin-3--glucoside being the majority phenolic component, followed by quercetin--pentosyl-hexoside and quercetin-dihexoside. This pioneering study, evidences that Tunisian okra display promising antioxidant and cytotoxic actions, in addition to relevant inhibitory effects against α-amylase and α-glucosidase enzymes, and interesting analgesic activity.
本研究旨在对突尼斯地方品种（Marsaouia）的辣木进行前所未有的深入研究。为此，对其营养成分、香气挥发物和酚类成分进行了分析，并评估了各种生物活性。
其近似成分表明，总膳食纤维是最丰富的宏量营养素，主要是不溶性膳食纤维，其次是总碳水化合物和蛋白质。此外，秋葵豆荚富含 K、Ca、Mg、有机酸、生育酚和叶绿素。气相色谱-电子轰击质谱（GC-EIMS）分析表明，氧代单萜、倍半萜烃和苯丙素是豆荚中主要的挥发性成分。通过高效液相色谱-二极管阵列检测器和电喷雾电离质谱（HPLC-DAD-MS/ESI）检测到八种类黄酮；以槲皮素-3-O-葡萄糖苷为主要酚类成分，其次是槲皮素-五糖基-己糖苷和槲皮素-二糖苷。这项开创性的研究表明，突尼斯秋葵具有良好的抗氧化和细胞毒性作用，同时对α-淀粉酶和α-葡萄糖苷酶具有相关抑制作用，以及有趣的镇痛活性。
