Avico Edgardo H, Acevedo Raúl M, Duarte María J, Rodrigues Salvador Acácio, Nunes-Nesi Adriano, Ruiz Oscar A, Sansberro Pedro A
Laboratorio de Biotecnología Aplicada y Genómica Funcional, Instituto de Botánica del Nordeste (IBONE-CONICET), Facultad de Ciencias Agrarias, Universidad Nacional del Nordeste, Sgto. Cabral 2131, Corrientes W3402BKG, Argentina.
National Institute of Science and Technology on Plant Physiology under Stress Conditions, Departamento de Biologia Vegetal, Universidade Federal de Viçosa, Viçosa 36570-900, MG, Brazil.
Plants (Basel). 2023 Jun 21;12(13):2404. doi: 10.3390/plants12132404.
The appearance of water stress episodes triggers leaf abscission and decreases yield. To explore the mechanisms that allow it to overcome dehydration, we investigated how the root gene expression varied between water-stressed and non-stressed plants and how the modulation of gene expression was linked to metabolite composition and physiological status. After water deprivation, 5160 differentially expressed transcripts were obtained through RNA-seq. The functional enrichment of induced transcripts revealed significant transcriptional remodelling of stress-related perception, signalling, transcription, and metabolism. Simultaneously, the induction of the enzyme 9-cis-expoxycarotenoid dioxygenase (NCED) transcripts reflected the central role of the hormone abscisic acid in this response. Consequently, the total content of amino acids and soluble sugars increased, and that of starch decreased. Likewise, osmotic adjustment and radical growth were significantly promoted to preserve cell membranes and water uptake. This study provides a valuable resource for future research to understand the molecular adaptation of plants under drought conditions and facilitates the exploration of drought-tolerant candidate genes.
水分胁迫事件的出现会引发叶片脱落并降低产量。为了探究其克服脱水的机制，我们研究了水分胁迫植物和非胁迫植物之间根系基因表达的差异，以及基因表达的调控与代谢物组成和生理状态之间的联系。水分剥夺后，通过RNA测序获得了5160个差异表达的转录本。诱导转录本的功能富集揭示了与胁迫相关的感知、信号传导、转录和代谢的显著转录重塑。同时，9-顺式环氧类胡萝卜素双加氧酶（NCED）转录本的诱导反映了激素脱落酸在这一反应中的核心作用。因此，氨基酸和可溶性糖的总含量增加，淀粉含量降低。同样，渗透调节和根系生长显著促进，以保护细胞膜和水分吸收。本研究为未来理解植物在干旱条件下的分子适应性研究提供了宝贵资源，并有助于探索耐旱候选基因。
