Tomasella Martina, Calderan Alberto, Mihelčič Alenka, Petruzzellis Francesco, Braidotti Riccardo, Natale Sara, Lisjak Klemen, Sivilotti Paolo, Nardini Andrea
Department of Life Sciences, University of Trieste, via L. Giorgieri 10, 34127 Trieste, Italy.
Department of Agricultural, Food, Environmental and Animal Sciences, University of Udine, Via delle Scienze 206, 33100 Udine, Italy.
Plants (Basel). 2023 Jun 22;12(13):2412. doi: 10.3390/plants12132412.
The pressure chamber is the most used tool for plant water status monitoring. However, species/cultivar and seasonal effects on protocols for reliable water potential determination have not been properly tested. In four grapevine cultivars and two times of the season (early season, Es; late season, Ls, under moderate drought), we assessed the maximum sample storage time before leaf water potential (Ψ) measurements and the minimum equilibration time for stem water potential (Ψ) determination, taking 24 h leaf cover as control. In 'Pinot gris', Ψ already decreased after 1 h leaf storage in both campaigns, dropping by 0.4/0.5 MPa after 3 h, while in 'Refosk', it decreased by 0.1 MPa after 1 and 2 h in Es and Ls, respectively. In 'Merlot' and 'Merlot Kanthus', even 3 h storage did not affect Ψ. In Es, the minimum Ψ equilibration was 1 h for 'Refošk' and 10 min for 'Pinot gris' and 'Merlot'. In Ls, 'Merlot Kanthus' required more than 2 h equilibration, while 1 h to 10 min was sufficient for the other cultivars. The observed cultivar and seasonal differences indicate that the proposed tests should be routinely performed prior to experiments to define ad hoc procedures for water status determination.
压力室是植物水分状况监测中使用最多的工具。然而，物种/品种和季节对可靠测定水势的方案的影响尚未得到充分测试。在四个葡萄品种以及季节中的两个时段（季节早期，Es；季节晚期，Ls，处于中度干旱条件下），我们以24小时叶片覆盖作为对照，评估了在测量叶片水势（Ψ）之前样品的最长储存时间以及测定茎水势（Ψ）所需的最短平衡时间。在“灰皮诺”品种中，在两个时段中，叶片储存1小时后Ψ就已下降，3小时后分别下降了0.4/0.5兆帕，而在“雷弗斯科”品种中，在Es时段和Ls时段，分别在1小时和2小时后下降了0.1兆帕。在“梅洛”和“梅洛坎图斯”品种中，即使储存3小时也不会影响Ψ。在Es时段，“雷弗斯科”的最短Ψ平衡时间为1小时，“灰皮诺”和“梅洛”为10分钟。在Ls时段，“梅洛坎图斯”需要超过2小时的平衡时间，而其他品种1小时到10分钟就足够了。观察到的品种和季节差异表明，在实验之前应常规进行所提议的测试，以确定用于水分状况测定的特定程序。
