He Dan, Zhang Dawei, Li Ting, Liu Lili, Zhou Dinggang, Kang Lei, Wu Jinfeng, Liu Zhongsong, Yan Mingli
School of Life Science, Hunan University of Science and Technology, Xiangtan, China.
Hunan Key Laboratory of Economic Crops Genetic Improvement and Integrated Utilization, Xiangtan, China.
Front Genet. 2021 Nov 18;12:764835. doi: 10.3389/fgene.2021.764835. eCollection 2021.
Anthocyanins contribute to most colors of plants and play protective roles in response to abiotic stresses. is widely cultivated worldwide as both an oilseed and a vegetable. However, only several high anthocyanin-containing cultivars have been reported, and the mechanisms of anthocyanin accumulation have not been well-elucidated in . Here, the phenotype, comparative whole-genome identification, and gene expression analysis were performed to investigate the dynamic change of the anthocyanin content and the gene expression patterns of anthocyanin biosynthetic genes (ABGs) in . A total of 152 ABGs were identified in the reference genome. To screen out the critical genes involved in anthocyanin biosynthesis and accumulation, the RNA-seq of young leaves of two lines with purple leaves (PL) or green leaves (GL), and their F progeny at 41, 91, and 101 days were performed to identify the differentially expressed genes. The comparative expression analysis of these ABGs indicated that the upregulation of together with its target genes (such as , , , and ) might promote the anthocyanin accumulation in PL at the early developmental stage (41-91 days). While the downregulation of those ABGs and anthocyanin degradation at the late developmental stage (91-101 days) might result in the decrease in anthocyanin accumulation. Our results would enhance the understanding of the regulatory network of anthocyanin dynamic accumulation in .
花青素赋予了植物大部分颜色，并在应对非生物胁迫时发挥保护作用。[植物名称]作为油料作物和蔬菜在全球广泛种植。然而，仅报道了几个富含花青素的品种，其花青素积累机制在[植物名称]中尚未得到充分阐明。在此，通过表型分析、全基因组比较鉴定和基因表达分析，研究了[植物名称]中花青素含量的动态变化以及花青素生物合成基因（ABGs）的基因表达模式。在[植物名称]参考基因组中总共鉴定出152个ABGs。为筛选出参与花青素生物合成和积累的关键基因，对两个具有紫色叶（PL）或绿色叶（GL）的[植物名称]品系及其F代在41、91和101天时的幼叶进行RNA测序，以鉴定差异表达基因。这些ABGs的比较表达分析表明，[基因名称]及其靶基因（如[基因名称1]、[基因名称2]、[基因名称3]和[基因名称4]）的上调可能在发育早期（41 - 91天）促进PL中花青素的积累。而在发育后期（91 - 101天）这些ABGs的下调和花青素降解可能导致花青素积累减少。我们的结果将增进对[植物名称]中花青素动态积累调控网络的理解。
