Department of Physics and Engineering Physics, Fordham University, Bronx, NY 10458, USA.
Sensors (Basel). 2023 Jun 26;23(13):5925. doi: 10.3390/s23135925.
Whispering gallery mode resonators have proven to be robust and sensitive platforms for the trace detection of chemical and/or biological analytes. Conventional approaches using serially addressed resonators face challenges in simultaneous multi-channel (i.e., multi-species) detection. We present an alternative monitoring scheme that allows for the spatial multiplexing of whispering gallery mode resonators with the simultaneous observation of the resonance spectra from each of them. By imaging arrays of microspheres and monitoring the glare spot intensities through image processing routines, resonance spectra from multiple resonators may be simultaneously recorded without interference or confounding effects of serial excitation/detection. We demonstrate our multiplexed imaging approach with bulk refractive index variations and virus-antibody binding.
whispering gallery 模式谐振器已被证明是用于痕量检测化学和/或生物分析物的稳健且灵敏的平台。使用串行寻址谐振器的传统方法在同时进行多通道（即多物种）检测方面面临挑战。我们提出了一种替代的监测方案，允许 whispering gallery 模式谐振器的空间复用，并同时观察它们各自的谐振光谱。通过对微球阵列进行成像并通过图像处理程序监测耀斑强度，可以在没有串扰或串行激发/检测的干扰或混淆效应的情况下同时记录来自多个谐振器的共振光谱。我们通过体折射率变化和病毒-抗体结合来演示我们的多路成像方法。
