Nielsen Signe M, Meyer Rikke L, Nørskov-Lauritsen Niels
Department of Clinical Medicine, Health, Aarhus University, DK-8200 Aarhus, Denmark.
Department of Clinical Microbiology, Aarhus University Hospital, DK-8200 Aarhus, Denmark.
Pathogens. 2017 May 19;6(2):20. doi: 10.3390/pathogens6020020.
Bacteria of genus are emerging pathogens in cystic fibrosis (CF) capable of biofilm formation and development of antimicrobial resistance. Evolutionary adaptions in the transition from primary to chronic infection were assessed by transcriptomic analysis of successive isolates of from a single CF patient. Several efflux pump systems targeting antimicrobial agents were upregulated during the course of the disease, whereas all genes related to motility were downregulated. Genes annotated to subsystems of sulfur metabolism, protein metabolism and potassium metabolism exhibited the strongest upregulation. K+ channel genes were hyperexpressed, and a putative sulfite oxidase was more than 1500 times upregulated. The transcriptome patterns indicated a pivotal role of sulfur metabolism and electrical signalling in biofilms during late stage CF lung disease.
属细菌是囊性纤维化（CF）中新兴的病原体，能够形成生物膜并产生抗菌耐药性。通过对一名CF患者连续分离的菌株进行转录组分析，评估了从原发性感染到慢性感染转变过程中的进化适应性。在疾病过程中，几种针对抗菌剂的外排泵系统上调，而所有与运动性相关的基因均下调。注释到硫代谢、蛋白质代谢和钾代谢子系统的基因上调最为明显。钾离子通道基因过度表达，一种假定的亚硫酸盐氧化酶上调超过1500倍。转录组模式表明，在CF晚期肺部疾病中，硫代谢和电信号在生物膜中起关键作用。
