Tyagi Saniya, Srinivasa Narayana, Singh R N, Vinay N
Department of Entomology and Agricultural Zoology, Institute of Agricultural Sciences, Banaras Hindu University, Varanasi, Uttar Pradesh 221005 India.
Department of Entomology, BRD PG College, Deoria, Uttar Pradesh 274001 India.
3 Biotech. 2023 Aug;13(8):269. doi: 10.1007/s13205-023-03693-x. Epub 2023 Jul 11.
Brown planthopper (BPH), (Stål) and white-backed planthopper (WBPH), (Horváth) are the most destructive sucking insect pests of rice in all rice growing parts of the world. For their accurate identification at early stages, we have developed two species-specific markers (SNL4F and SNL4R for BPH; SNF2F and SNF2R for WBPH) based on mitochondrial cytochrome oxidase I (COI) for their easy detection using Polymerase Chain Reaction (PCR). The markers were developed based on nucleotide differences in COI gene and were subjected to various tests based on PCR-based gel images. The designed primers were cross-checked with five other species, which confirmed their specificity. The primers were also found to be efficient in identification of their respective species (BPH and WBPH) in all the individuals sampled from different regions of India. The lowest detection sensitivity of both the primers was up to 1 ng/µl DNA after testing them through a series of varied DNA concentrations. The species-specific primers developed in this study will help in easy and rapid identification of BPH and WBPH in all the stages of their development and in turn facilitate their timely management.
The online version contains supplementary material available at 10.1007/s13205-023-03693-x.
褐飞虱（Nilaparvata lugens，（斯塔尔））和白背飞虱（Sogatella furcifera，（霍瓦特））是世界上所有水稻种植区最具破坏性的刺吸式害虫。为了在早期阶段对它们进行准确识别，我们基于线粒体细胞色素氧化酶I（COI）开发了两种物种特异性标记（用于褐飞虱的SNL4F和SNL4R；用于白背飞虱的SNF2F和SNF2R），以便使用聚合酶链反应（PCR）轻松检测。这些标记是基于COI基因的核苷酸差异开发的，并根据基于PCR的凝胶图像进行了各种测试。设计的引物与其他五个物种进行了交叉检查，证实了它们的特异性。还发现这些引物在识别从印度不同地区采集的所有个体中的各自物种（褐飞虱和白背飞虱）方面是有效的。通过一系列不同的DNA浓度对引物进行测试后，两种引物的最低检测灵敏度均高达1 ng/µl DNA。本研究中开发的物种特异性引物将有助于在褐飞虱和白背飞虱发育的所有阶段轻松快速地识别它们，进而促进对它们的及时管理。
在线版本包含可在10.1007/s13205-023-03693-x获取的补充材料。
