用于动物模型饲养试验的糖化牛血清白蛋白 - 糖化底物用于饲料颗粒改性的体外方法和特性。

Glycated bovine serum albumin for use in feeding trials with animal models - In vitro methodology and characterization of a glycated substrate for modifying feed pellets.

机构信息

U1167-RID-AGE-Facteurs de Risque et Déterminants Moléculaires des Maladies Liées au Vieillissement, Institut Pasteur de Lille, University Lille, Inserm, CHU Lille, F-59000 Lille, France.

Institut Polytechnique UniLaSalle, Université d'Artois, ULR 7519, 60000 Beauvais, France.

出版信息

Food Chem. 2023 Dec 1;428:136815. doi: 10.1016/j.foodchem.2023.136815. Epub 2023 Jul 4.

DOI:10.1016/j.foodchem.2023.136815

PMID:37450953

Abstract

This study investigated different methods to produce N-carboxymethyl-lysine (CML)-enriched bovine serum albumin (BSA) as alternatives to the classical approach using glyoxylic acid (GA) and sodium cyanoborohydride (NaBHCN) which results in toxic hydrogen cyanide (HCN). The reaction of GA (6 mmol/L) and NaBHCN (21 mmol/L) to produce CML remained the most effective with CML yields of 24-35%, followed by 13-24% using 300 mmol/L glyoxal (GO). GA promoted specific modification of lysine to CML, and fewer structural modifications of the BSA molecule compared with GO, as evidenced by fluorescence and proteomic analyses. GO promoted greater arginine modification compared with GA (76 vs 23%). Despite structural changes to BSA with GO, murine fecal clearance of CML was similar to literature values. Hence, BSA glycation with 300 mmol/L glyoxal is a suitable alternative to GA and NaBHCN for generating CML-enriched protein free of HCN, but a CML-only fortification model remains to be described.

摘要

本研究调查了不同的方法来生产 N-羧甲基赖氨酸（CML）富集的牛血清白蛋白（BSA），以替代使用乙二醛（GA）和氰基硼氢化钠（NaBHCN）的经典方法，因为后者会产生有毒的氰化氢（HCN）。GA（6 mmol/L）和 NaBHCN（21 mmol/L）反应生成 CML 的效果仍然最有效，CML 产率为 24-35%，其次是使用 300 mmol/L 乙二醛（GO）的 13-24%。GA 促进赖氨酸特异性修饰为 CML，与 GO 相比，BSA 分子的结构修饰较少，荧光和蛋白质组学分析证明了这一点。GO 促进了比 GA 更多的精氨酸修饰（76 比 23%）。尽管 GO 会导致 BSA 的结构发生变化，但 CML 在小鼠粪便中的清除率与文献值相似。因此，用 300 mmol/L 乙二醛对 BSA 进行糖化是一种替代 GA 和 NaBHCN 生成不含 HCN 的 CML 富集蛋白的合适方法，但仍需要描述 CML 强化模型。