通过疏水相互作用色谱法揭示的用于评估细菌疏水性的生物发光测定法。
Bioluminescence assay for estimating the hydrophobic properties of bacteria as revealed by hydrophobic interaction chromatography.
作者信息
Mafu A A, Roy D, Savoie L, Goulet J
机构信息
Agriculture Canada Food Research and Development Centre, St.-Hyacinthe, Quebec.
出版信息
Appl Environ Microbiol. 1991 Jun;57(6):1640-3. doi: 10.1128/aem.57.6.1640-1643.1991.
Abstract
The luciferin-luciferase bioluminescence method was used to estimate the number of bacteria retained in neutral and amphiphilic gels and those in the eluate to determine the hydrophobic surface properties of bacteria by using hydrophobic interaction chromatography. Good correlations were found between viable counts and ATP content for Escherichia coli, Pseudomonas fragi, and Listeria monocytogenes. ATP determination was more rapid than viable counts for characterizing the relative hydrophobicity of L. monocytogenes. Quantitative estimations of adsorption of L. monocytogenes on octyl-Sepharose indicate that this microorganism is hydrophilic.
摘要
采用荧光素-荧光素酶生物发光法，通过疏水相互作用色谱法来估算保留在中性和两亲性凝胶中的细菌数量以及洗脱液中的细菌数量，以确定细菌的疏水表面特性。对于大肠杆菌、脆弱拟杆菌和单核细胞增生李斯特菌，发现活菌计数与ATP含量之间具有良好的相关性。在表征单核细胞增生李斯特菌的相对疏水性方面，ATP测定比活菌计数更快。对单核细胞增生李斯特菌在辛基琼脂糖上的吸附进行定量估计表明，这种微生物是亲水的。
相似文献
1
Bioluminescence assay for estimating the hydrophobic properties of bacteria as revealed by hydrophobic interaction chromatography.通过疏水相互作用色谱法揭示的用于评估细菌疏水性的生物发光测定法。
Appl Environ Microbiol. 1991 Jun;57(6):1640-3. doi: 10.1128/aem.57.6.1640-1643.1991.
2
Using ATP bioluminescence technique for monitoring microbial activity in sludge.使用ATP生物发光技术监测污泥中的微生物活性。
Biotechnol Bioeng. 2001 Nov 20;75(4):469-74. doi: 10.1002/bit.10076.
3
Rapid diagnosis of bacteraemia by measuring bacterial adenosine triphosphate in blood culture bottles using bioluminescence.
Med Microbiol Immunol. 1983;172(2):117-22. doi: 10.1007/BF02124512.
4
A Microfluidic Device for Rapid Screening of E. coli O157:H7 Based on IFAST and ATP Bioluminescence Assay for Water Analysis.一种基于IFAST和ATP生物发光分析法用于水分析的快速筛选大肠杆菌O157:H7的微流控装置。
Chemistry. 2017 Sep 18;23(52):12754-12757. doi: 10.1002/chem.201703487. Epub 2017 Aug 18.
5
Performance characteristics of a new photometer with a moving filter tape for luminescence assay.一种带有可移动滤光带的新型光度计用于发光测定的性能特征。
Appl Environ Microbiol. 1977 Dec;34(6):720-4. doi: 10.1128/aem.34.6.720-724.1977.
6
Assessment of photodynamic destruction of Escherichia coli O157:H7 and Listeria monocytogenes by using ATP bioluminescence.利用ATP生物发光法评估光动力对大肠杆菌O157:H7和单核细胞增生李斯特菌的破坏作用。
Appl Environ Microbiol. 2003 Nov;69(11):6393-8. doi: 10.1128/AEM.69.11.6393-6398.2003.
7
The enumeration of Leptospira interrogans serovar pomona by a bioluminescence ATP assay.通过生物发光ATP检测法对波摩那群问号钩端螺旋体进行计数。
J Biol Stand. 1986 Jan;14(1):21-4. doi: 10.1016/s0092-1157(86)80005-1.
8
Biosynthesis-inspired deracemizative production of d-luciferin by combining luciferase and thioesterase.受生物合成启发，通过组合荧光素酶和硫酯酶实现 d-荧光素的外消旋化生产。
Biochim Biophys Acta Gen Subj. 2017 Aug;1861(8):2112-2118. doi: 10.1016/j.bbagen.2017.04.010. Epub 2017 Apr 25.
9
Characterization of physicochemical forces involved in adhesion of Listeria monocytogenes to surfaces.单核细胞增生李斯特菌黏附于表面所涉及的物理化学力的表征
Appl Environ Microbiol. 1991 Jul;57(7):1969-73. doi: 10.1128/aem.57.7.1969-1973.1991.
10
Kinetics of bactericidal activity of antibiotics measured by luciferin-luciferase assay.通过荧光素-荧光素酶测定法测量抗生素杀菌活性的动力学。
J Biolumin Chemilumin. 1991 Jul-Sep;6(3):193-201. doi: 10.1002/bio.1170060310.
引用本文的文献
1
An Overview on Novel Microbial Determination Methods in Pharmaceutical and Food Quality Control.制药与食品质量控制中新型微生物检测方法概述
Adv Pharm Bull. 2016 Sep;6(3):301-308. doi: 10.15171/apb.2016.042. Epub 2016 Sep 25.
2
Characterization of physicochemical forces involved in adhesion of Listeria monocytogenes to surfaces.单核细胞增生李斯特菌黏附于表面所涉及的物理化学力的表征
Appl Environ Microbiol. 1991 Jul;57(7):1969-73. doi: 10.1128/aem.57.7.1969-1973.1991.
本文引用的文献
1
Bioluminescence Assay for Measuring the Number of Bacteria Adhering to the Hydrocarbon Phase in the BATH Test.用于测量 BATH 试验中附着在烃相上的细菌数量的生物发光测定法。
Appl Environ Microbiol. 1988 Jun;54(6):1436-9. doi: 10.1128/aem.54.6.1436-1439.1988.
2
[Firefly assay of bacterial ATP: comparative study of extraction methods (author's transl)].[细菌ATP的萤火虫荧光素酶检测法：提取方法的比较研究（作者译）]
Ann Biol Clin (Paris). 1980;38(5):297-304.
3
The hydrophobicity of bacteria - an important factor in their initial adhesion at the air-water interface.细菌的疏水性——其在气-水界面初始黏附的一个重要因素。
Arch Microbiol. 1981 Jan;128(3):267-70. doi: 10.1007/BF00422527.
4
Cellular nucleotide measurements and applications in microbial ecology.细胞核苷酸测量及其在微生物生态学中的应用。
Microbiol Rev. 1980 Dec;44(4):739-96. doi: 10.1128/mr.44.4.739-796.1980.
5
Hydrophobic interaction--a mechanism of bacterial binding.
Scand J Infect Dis Suppl. 1982;33:32-6.
6
Estimation of Campylobacter spp. in broth culture by bioluminescence assay of ATP.通过ATP生物发光测定法估算肉汤培养物中的弯曲杆菌属。
Appl Environ Microbiol. 1985 Mar;49(3):730-1. doi: 10.1128/aem.49.3.730-731.1985.
7
Determination of hydrophobicity on bacterial surfaces by nonionic surfactants.非离子表面活性剂对细菌表面疏水性的测定。
J Bacteriol. 1986 Sep;167(3):1016-9. doi: 10.1128/jb.167.3.1016-1019.1986.
8
Kinetics of Pseudomonas aeruginosa adhesion to 304 and 316-L stainless steel: role of cell surface hydrophobicity.铜绿假单胞菌对304和316-L不锈钢的黏附动力学：细胞表面疏水性的作用
Appl Environ Microbiol. 1990 Mar;56(3):788-95. doi: 10.1128/aem.56.3.788-795.1990.
9
Adenosine triphosphate pool levels and endogenous metabolism in Arthrobacter crystallopoietes during growth and starvation.产晶体节杆菌在生长和饥饿期间的三磷酸腺苷池水平及内源性代谢
Arch Microbiol. 1979 Jul;122(1):61-7. doi: 10.1007/BF00408047.
10
Differences in hydrophobic surface characteristics of porcine enteropathogenic Escherichia coli with or without K88 antigen as revealed by hydrophobic interaction chromatography.通过疏水相互作用色谱法揭示的具有或不具有K88抗原的猪肠道致病性大肠杆菌疏水表面特征的差异
Infect Immun. 1978 Nov;22(2):462-72. doi: 10.1128/iai.22.2.462-472.1978.
Zotero 插件