Universiti Kuala Lumpur, Branch Campus Institute of Medical Science Technology, A1, 1, Jalan TKS 1, Taman Kajang Sentral, 43000, Kajang, Selangor, Malaysia; Universiti Kuala Lumpur, Branch Campus Malaysian Institute of Chemical and Bioengineering Technology, Lot 1988 Kawasan Perindustrian Bandar Vendor, Taboh Naning, Alor Gajah, Melaka, Malaysia.
School of Distance Education, Universiti Sains Malaysia, 11800, Minden, Penang, Malaysia.
Chemosphere. 2023 Oct;338:139492. doi: 10.1016/j.chemosphere.2023.139492. Epub 2023 Jul 12.
Vancomycin is the last resort antibiotic for the treatment of severe bacterial keratitis. Its clinical application is limited due to its hydrophilicity and high molecular weight. To overcome this, this study aims to develop nanoparticles-laden contact lens for controlled ocular delivery of vancomycin. Polyvinyl alcohol (PVA) was used as encapsulant material. The nanoparticles had a negative surface charge and an average size of 147.6 nm. A satisfactory encapsulation efficiency (61.24%) was obtained. The release profile was observed to be slow and sustained, with a release rate of 1.29 μL mg h for 48 h. Five out of 6 test bacteria were suppressed by vancomycin nanoparticles-laden contact lens. Vancomycin is generally ineffective against Gram-negative bacteria and unable to pass through the outer membrane barrier. In this study, vancomycin inhibited Proteus mirabilis and Pseudomonas aeruginosa. Nano-encapsulation enables vancomycin to penetrate the Gram-negative cell wall and further destroy the bacterial cells. On Hohenstein challenge test, all test bacteria exhibited significant reduction in growth when exposed to vancomycin nanoparticles-laden contact lens. This study created an effective and long-lasting vancomycin delivery system via silicone hydrogel contact lenses, by using PVA as encapsulant. The antibiotic efficacy and vancomycin release should be further studied using ocular in vivo models.
万古霉素是治疗严重细菌性角膜炎的最后一线抗生素。由于其亲水性和高分子量，其临床应用受到限制。为了克服这一问题，本研究旨在开发载有纳米颗粒的隐形眼镜，以实现万古霉素的眼部控制释放。聚乙烯醇（PVA）被用作包封材料。纳米颗粒带负电荷，平均粒径为 147.6nm。获得了令人满意的包封效率（61.24%）。释放曲线呈缓慢持续释放，48 小时内释放率为 1.29μLmg h。载有万古霉素纳米颗粒的隐形眼镜抑制了 6 株测试菌中的 5 株。万古霉素通常对革兰氏阴性菌无效，且无法穿透外膜屏障。在本研究中，万古霉素抑制了奇异变形杆菌和铜绿假单胞菌。纳米封装使万古霉素能够穿透革兰氏阴性细胞壁，并进一步破坏细菌细胞。在 Hohenstein 挑战试验中，所有测试菌在接触载有万古霉素纳米颗粒的隐形眼镜时，其生长均显著减少。本研究通过硅水凝胶隐形眼镜成功开发了一种有效的、长效的万古霉素递送系统，使用 PVA 作为包封材料。应使用眼部体内模型进一步研究抗生素功效和万古霉素释放。
