Genome characteristics of the -positive strain QHY-2 carrying a novel plasmid type.

is a bacterial species of importance to both public and animal health. The gene is the first gene that confers resistance to the tedizolid, a last-resort antimicrobial agent in human medicine. Herein, we whole-genome sequenced and analyzed one -positive strain QHY-2 from Tibetan sheep in Qinghai province and identified one plasmid pQHY-2. The plasmid shared similar structure with the -positive plasmids p2C45 and p21-D-5b previously identified in , demonstrating the potential horizontal transmission of the plasmids among strains. Annotation of the -positive plasmids showed and (A) located on a segment flanked by IS element IS, and , and (A) located on a segment flanked by IS element IS, which revealed the possible dissemination mechanism. Additionally, a Tn-like transposon carrying ) and (B) was also detected on pQHY-2, demonstrating the transposition of Tn and spread of antibiotic resistance among bacteria. Molecular analysis indicated the -positive plasmids belonged to a plasmid type distinct from the pCW3-like plasmids, pCP13-like plasmids, or pIP404-like plasmids. Further structure analysis showed they might be formed by inserting segments into plasmid pCPCPI53k-r1_1, which coexist with two pCW3-like plasmids and one pCP13-like plasmid in strain CPI 53k-r1 isolated from a healthy human in Finland. IMPORTANCE Antimicrobial resistance is now a global concern posing threats to food safety and public health. The pCW3-like plasmids can encode several main toxin genes and three antibiotic resistance genes (ARGs), including (P), (P), and (B), which used to be considered as the main carrier of ARGs in . In this study, we found the plasmids, which belonged to a novel plasmid type, could also harbor many other ARGs, indicating this type of plasmid might be the potential repository of ARGs in . Additionally, this type of plasmid could coexist with the pCW3-like plasmids and pCP13-like plasmids that encoded toxin genes associated with gastrointestinal diseases, which showed the potential threat to public health.