一种多维狭缝扫描流动系统。

A multidimensional slit-scan flow system.

作者信息

Cambier J L, Kay D B, Wheeless L L

出版信息

J Histochem Cytochem. 1979 Jan;27(1):321-4. doi: 10.1177/27.1.374595.

DOI:10.1177/27.1.374595

Abstract

A new slit-scan type flow system is described which provides three (X, Y, and Z) orthogonal one-dimensional projections of cell fluorescence. A photomultiplier tube and two semiconductor array detectors are used to obtain the three slit-scan contours from cells traversing a single fluorescence excitation beam. A high speed, dedicated preprocessor analyzes the three contours in parallel, extracting certain features useful for rejecting cells from which an accurate measurement of nuclear fluorescence cannot be obtain. Contour data is buffered and transferred to a PDP-11/40 computer where nuclear fluorescence is measured and cells are classified. It is anticipated that this new instrument will provide a significant reduction in false alarm rate when applied to prescreening of gynecologic cytology specimens.

摘要

本文描述了一种新型的狭缝扫描式流动系统，该系统可提供细胞荧光的三个（X、Y和Z）正交一维投影。使用一个光电倍增管和两个半导体阵列探测器，从穿过单个荧光激发光束的细胞中获取三个狭缝扫描轮廓。一个高速专用预处理器并行分析这三个轮廓，提取某些有助于排除无法获得核荧光准确测量值的细胞的有用特征。轮廓数据被缓冲并传输到一台PDP-11/40计算机，在那里测量核荧光并对细胞进行分类。预计这种新仪器应用于妇科细胞学标本的预筛查时，将显著降低误报率。