DPV UL41 基因编码的蛋白诱导宿主关闭活性并影响病毒复制。

DPV UL41 gene encoding protein induces host shutoff activity and affects viral replication.

机构信息

Institute of Preventive Veterinary Medicine, Sichuan Agricultural University, Wenjiang, Chengdu City, Sichuan, 611130, PR China; Key Laboratory of Animal Disease and Human Health of Sichuan Province, Sichuan Agricultural University, Wenjiang, Chengdu City, Sichuan, 611130, PR China; Avian Disease Research Center, College of Veterinary Medicine, Sichuan Agricultural University, Wenjiang, Chengdu City, Sichuan, 611130, PR China.

出版信息

Vet Microbiol. 2021 Apr;255:108979. doi: 10.1016/j.vetmic.2021.108979. Epub 2021 Feb 27.

DOI:10.1016/j.vetmic.2021.108979

PMID:33721633

Abstract

The virion host shutoff (VHS) protein, encoded by the UL41 gene of herpes simplex virus (HSV), specifically degrades mRNA and induces host shutoff. VHS and its homologs are highly conserved in the Alphaherpesvirinae subfamily. However, the role of the duck plague virus (DPV) UL41 gene is unclear. In this study, we found that the DPV UL41 gene-encoded protein (pUL41) degrades RNA polymerase (pol) II-transcribed translatable RNA and induces protein synthesis shutoff. DPV pUL41 was dispensable for viral replication, but the UL41-deleted mutant virus exhibited a significant viral growth defect and plaque size reduction in Duck embryo fibroblast (DEF) cells. Furthermore, DPV pUL41 regulated viral mRNA accumulation to affect viral DNA replication, release and cell-to-cell spread.

摘要

病毒粒子宿主关闭 (VHS) 蛋白，由单纯疱疹病毒 (HSV) 的 UL41 基因编码，特异性降解 mRNA 并诱导宿主关闭。VHS 及其同源物在α疱疹病毒亚科中高度保守。然而，鸭瘟病毒 (DPV) UL41 基因的作用尚不清楚。在本研究中，我们发现 DPV UL41 基因编码的蛋白 (pUL41) 降解 RNA 聚合酶 (pol) II 转录的可翻译 RNA 并诱导蛋白质合成关闭。DPV pUL41 对于病毒复制不是必需的，但是 UL41 缺失突变病毒在鸭胚成纤维细胞 (DEF) 中表现出明显的病毒生长缺陷和蚀斑大小减小。此外，DPV pUL41 调节病毒 mRNA 积累以影响病毒 DNA 复制、释放和细胞间传播。