von Sengbusch G, Achatz M, Beck R, Betz J W, Härtel W, Mann R, Bader P, Schlüter G, Seger G
J Histochem Cytochem. 1979 Jan;27(1):600-3. doi: 10.1177/27.1.374627.
In this report, a preselection of alarms in a system for automated screening of cervical cancer based on depositing the cell sample linearly as a "cell trace" on a tape and analyzing it at different decision levels with increasing complexity, and preliminary results on analyzing cervical material with this system are discussed. The "cell trace" is analyzed with the slit-scan technique. Six parameters are computed: 1) cellular diameter; 2) nuclear diameter; 3) nuclear fluorescence (acriflavin-Feulgen) as nuclear DNA; 4) cellular fluorescence; 5) nuclear to cytoplasm ratio (N/C ratio); and 6) nuclear density. At present, only nuclear fluorescence is used to define a decision boundary between normal and potentially atypical cells. Under this criteria the slit-scan analysis leaves 5% of the events in a sample that must be rechecked at a second decision level in normal cell samples. A further reduction is expected when several slit-scan parameters are used at the first decision step. All events declared suspicious will be investigated in more detail by a two dimensional image analyzing system where the fluorescence image is generated by a laser scanning system. Results obtained in preliminary experiments are discussed in this paper.
在本报告中，讨论了一种用于宫颈癌自动筛查系统中的警报预筛选方法，该系统通过将细胞样本作为“细胞轨迹”线性沉积在磁带上，并在不同决策水平上以递增的复杂度进行分析，同时还讨论了使用该系统分析宫颈材料的初步结果。采用缝隙扫描技术对“细胞轨迹”进行分析。计算六个参数：1）细胞直径；2）细胞核直径；3）作为核DNA的核荧光（吖啶黄素-福尔根）；4）细胞荧光；5）核质比（N/C比）；6）核密度。目前，仅使用核荧光来定义正常细胞和潜在非典型细胞之间的决策边界。根据该标准，缝隙扫描分析在正常细胞样本中会留下5%的事件，这些事件必须在第二个决策水平上重新检查。当在第一个决策步骤中使用多个缝隙扫描参数时，预计会有进一步的减少。所有被判定为可疑的事件将通过二维图像分析系统进行更详细的调查，其中荧光图像由激光扫描系统生成。本文讨论了初步实验中获得的结果。
