Al I, Ploem J S
J Histochem Cytochem. 1979 Jan;27(1):629-34. doi: 10.1177/27.1.86580.
In slide based automation of cervical cytology the first stage of analysis involves finding possibly suspicious cells, or areas on the slide with these types of cells. By using a television based system such as the Leyden Television Analysis System (LEYTAS), a number of detection methods can be applied to rapidly screen a large number of fields automatically for suspicious cells. In this paper, results using a parameter based on increased nuclear DNA content of cells are given and a second detection method based on a chromatin pattern feature, called chromatin contrast, is discussed. Two blind trials on 41 positive and 22 negative cervical slides, using the Leyden Television Analysis System to detect suspicious cells with an increased nuclear DNA content, were promising. In 1 of the 41 positive cases no suspicious cells were found. In the negative specimens, suspicious cells were detected in 1 of 9 cases and 1 of 13 cases, with the two detection parameters investigated. These findings are discussed and some automatic artefact rejection procedures with preliminary results are given.
在基于玻片的宫颈细胞学自动化分析中，第一阶段的分析工作是寻找可能可疑的细胞，或者玻片上带有这类细胞的区域。通过使用基于电视的系统，如莱顿电视分析系统（LEYTAS），可以应用多种检测方法自动快速筛查大量视野，以寻找可疑细胞。本文给出了基于细胞中核DNA含量增加这一参数的检测结果，并讨论了基于一种染色质模式特征（称为染色质对比度）的第二种检测方法。使用莱顿电视分析系统，对41张阳性和22张阴性宫颈玻片进行了两项盲法试验，以检测核DNA含量增加的可疑细胞，试验结果很有前景。在41例阳性病例中，有1例未发现可疑细胞。在阴性标本中，在9例中的1例和13例中的1例检测到了可疑细胞，这是在研究了两个检测参数的情况下得出的结果。本文对这些发现进行了讨论，并给出了一些具有初步结果的自动伪像排除程序。
