Department of Emergency, Beijing Tongren Hospital (South District), Capital Medical University, No. 2, Xihuan South Road, Daxing District, Beijing, 100176, China.
Ir J Med Sci. 2024 Feb;193(1):131-138. doi: 10.1007/s11845-023-03466-8. Epub 2023 Jul 19.
Acute coronary syndrome (ACS) is an emergency and severe disorder of the cardiovascular system. This paper assessed the expression of plasma HAND2-AS1 in patients with ACS, researched its diagnostic and prognostic significance, and studied its possible mechanism for participating in ACS.
The concentration of HAND2-AS1 of 101 included patients with ACS was certified by qRT-PCR and its possible diagnostic function was revealed by the receiver operating characteristic (ROC) curve. All patients were followed up for 6 months after percutaneous coronary intervention (PCI) therapy and Kaplan-Meier (K-M) curve and COX regression analysis was performed to estimate the short-term prognostic value of HAND2-AS1 in ACS. The interrelationship between HAND2-AS1 and Gensini score and endothelial injury was identified via Pearson correlation. The function of HAND2-AS1 on the viability, migration, and apoptosis of human umbilical vein endothelial cells (HUVECs) was estimated by the Cell Counting Kit-8 (CCK-8), Transwell chamber, and flow cytometry.
In ACS patients, the expression of serum HAND2-AS1 was prominently decreased and closely correlated with the Gensini score. The decreased HAND2-AS1 expression was of diagnostic significance. Declined plasma HAND2-AS1 was observed in patients with the major adverse cardio-cerebrovascular event (MACCE) and was an independent risk for the poor prognosis of ACS patients. In the cell model, upregulation of HAND2-AS1 improved cell viability and migration and inhibited cell apoptosis.
HAND2-AS1 was an independent biomarker for the diagnosis and prognosis of ACS. HAND2-AS1 might be involved in ACS development by regulating endothelial damage.
急性冠状动脉综合征（ACS）是一种心血管系统的紧急和严重疾病。本文评估了血浆 HAND2-AS1 在 ACS 患者中的表达，研究了其诊断和预后意义，并研究了其可能参与 ACS 的机制。
通过 qRT-PCR 验证了 101 例 ACS 患者的 HAND2-AS1 浓度，并通过接收者操作特征（ROC）曲线揭示了其可能的诊断功能。所有患者在经皮冠状动脉介入治疗（PCI）后均进行了 6 个月的随访，通过 Kaplan-Meier（K-M）曲线和 COX 回归分析评估 HAND2-AS1 在 ACS 中的短期预后价值。通过 Pearson 相关性分析确定 HAND2-AS1 与 Gensini 评分和内皮损伤之间的相互关系。通过细胞计数试剂盒-8（CCK-8）、Transwell 室和流式细胞术评估 HAND2-AS1 对人脐静脉内皮细胞（HUVEC）活力、迁移和凋亡的功能。
在 ACS 患者中，血清 HAND2-AS1 的表达明显降低，与 Gensini 评分密切相关。HAND2-AS1 表达降低具有诊断意义。在主要不良心脑血管事件（MACCE）患者中观察到降低的血浆 HAND2-AS1，并且是 ACS 患者预后不良的独立危险因素。在细胞模型中，上调 HAND2-AS1 可提高细胞活力和迁移，抑制细胞凋亡。
HAND2-AS1 是 ACS 诊断和预后的独立生物标志物。HAND2-AS1 可能通过调节内皮损伤参与 ACS 的发展。
