Grupo de Inmunobiología y Biología Celular, Pontificia Universidad Javeriana, Carrera 7a. No. 43-82, Ed. 50, Lab. 101, Bogotá, C.P. 110211, Colombia.
Grupo de Investigación en Fitoquímica, Pontificia Universidad Javeriana, Bogotá, Colombia.
Cancer Immunol Immunother. 2023 Oct;72(10):3279-3292. doi: 10.1007/s00262-023-03487-3. Epub 2023 Jul 18.
Although the antitumor effect of P. nigrum has been widely studied, research related to its possible immunomodulatory effects is relatively scarce. Here, the antitumor and immunomodulatory activity of an ethanolic extract of P. nigrum were evaluated in the murine models of 4T1 breast cancer and B16-F10 melanoma. In vitro evaluations showed that the P. nigrum extract has cytotoxic activity, induces apoptotic cell death, and has a pro-oxidant effect in both cell lines, but it regulates glucose uptake differently in both lines, decreasing it in 4T1 but not in B16-F10. P. nigrum extract significantly reduced tumor size in both models and decreased the occurrence of macrometastases in 4T1 model. Evaluation of immune subpopulations by flow cytometry revealed that the P. nigrum extract significantly increases the frequency of dendritic cells and activated CD8 T cells and decreases the frequency of myeloid-derived suppressor like cells and Tregs in the tumor microenvironment of both models but with different dynamics. Our findings strongly suggest that the P. nigrum extract exerts immunomodulatory functions, slightly related to the modulation of cellular energy metabolism, which could ultimately contribute to the promising antitumor effect of P. nigrum.
虽然黑枸杞的抗肿瘤作用已被广泛研究，但关于其可能的免疫调节作用的研究相对较少。在这里，我们评估了黑枸杞醇提物在 4T1 乳腺癌和 B16-F10 黑色素瘤小鼠模型中的抗肿瘤和免疫调节活性。体外评价表明，黑枸杞提取物在两种细胞系中均具有细胞毒性、诱导细胞凋亡和促氧化作用，但它在两种细胞系中对葡萄糖摄取的调节作用不同，在 4T1 中降低而在 B16-F10 中不降低。黑枸杞提取物在两种模型中均显著减小肿瘤体积，并减少 4T1 模型中巨转移的发生。通过流式细胞术评估免疫亚群表明，黑枸杞提取物显著增加了两种模型肿瘤微环境中树突状细胞和激活的 CD8 T 细胞的频率，降低了髓系来源的抑制性细胞和 Tregs 的频率，但动态不同。我们的研究结果强烈表明，黑枸杞提取物具有免疫调节功能，与细胞能量代谢的调节略有相关，这可能最终有助于黑枸杞的抗肿瘤作用。
