Department of Restorative Dentistry, Faculty of Dentistry of Araraquara, State University of São Paulo, Araraquara, São Paulo, Brazil.
Int Endod J. 2010 Jun;43(6):528-35. doi: 10.1111/j.1365-2591.2010.01712.x.
To investigate the antibacterial effect of Tetraclean, MTAD and five experimental irrigants using both direct exposure test with planktonic cultures and mixed-species in vitro biofilm model.
Tetraclean, MTAD and five experimental solutions that were modifications of existing formulae including MTAD + 0.01% cetrimide (CTR), MTAD + 0.1% CTR, MTAC-1 (Tween 80 replaced by 0.01% CTR in MTAD), MTAC-2 (Tween 80 replaced by 0.1% CTR) and MTAD-D (MTAD without the Tween 80 and no CTR added) were used as disinfectants in the experiments. In the direct exposure test, a suspension of Enterococcus faecalis was mixed with each of the solutions. After 0.5, 1, 3 and 10 min, an inactivator was added and the number of surviving bacteria was calculated. A mixed-species biofilm from subgingival plaque bacteria was grown in brain heart infusion broth in anaerobic conditions on synthetic hydroxyapatite discs. Two-week-old biofilms were exposed to the solutions for 0.5, 1 and 3 min. The samples were observed by confocal laser scanning microscopy after bacterial viability staining. The scans were quantitatively analysed, and the volume of killed cells of all cells was calculated for each medicament.
Tetraclean and MTAC-2 (0.1% CTR) killed planktonic E. faecalis in <30 s. Complete killing of bacteria required 1 min by MTAC-1, 3 min by MTAD + 0.1% CTR and 10 min by MTAD, MTAD-D and MTAD + 0.01% CTR. In the biofilm test, there were significant differences in microbial killing between the different solutions and times of exposure (P < 0.005). MTAC-2 showed the best performance, killing 71% of the biofilm bacteria in 3 min, followed by MTAC-1 and Tetraclean. MTAD and the three MTAD modifications demonstrated the lowest antibacterial activity.
Tetraclean was more effective than MTAD against E. faecalis in planktonic culture and in mixed-species in vitro biofilm. CTR improved the antimicrobial properties of the solutions, whereas Tween 80 seemed to have a neutral or negative impact on their antimicrobial effectiveness.
通过浮游培养物直接暴露试验和混合物种体外生物膜模型，研究特可清、MTAD 和五种实验冲洗液的抗菌效果。
特可清、MTAD 和五种实验溶液（包括 MTAD+0.01%西曲溴铵（CTR）、MTAD+0.1%CTR、MTAC-1（Tween 80 用 0.01%CTR 替代 MTAD）、MTAC-2（Tween 80 用 0.1%CTR 替代）和 MTAD-D（不含 Tween 80 且未添加 CTR 的 MTAD））用作实验中的消毒剂。在直接暴露试验中，将粪肠球菌悬浮液与每种溶液混合。0.5、1、3 和 10 分钟后，加入灭活剂并计算存活细菌数量。在无氧条件下，在脑心浸液肉汤中，在合成羟磷灰石盘上培养龈下菌斑细菌的混合物种生物膜。用 0.5、1 和 3 分钟暴露于溶液中培养两周的生物膜。用细菌活力染色后，用共聚焦激光扫描显微镜观察样品。对扫描结果进行定量分析，计算每种药物的所有细胞中被杀灭细胞的体积。
特可清和 MTAC-2（0.1%CTR）在<30 秒内杀死浮游状态的粪肠球菌。MTAC-1 需 1 分钟、MTAD+0.1%CTR 需 3 分钟、MTAD、MTAD-D 和 MTAD+0.01%CTR 需 10 分钟才能完全杀死细菌。在生物膜试验中，不同溶液和暴露时间之间的微生物杀灭效果存在显著差异（P<0.005）。MTAC-2 在 3 分钟内表现出最佳的杀菌性能，可杀死 71%的生物膜细菌，其次是 MTAC-1 和特可清。MTAD 和三种 MTAD 改性剂显示出最低的抗菌活性。
特可清对浮游培养物和混合物种体外生物膜中的粪肠球菌的效果优于 MTAD。CTR 提高了溶液的抗菌性能，而 Tween 80 似乎对其抗菌效果产生中性或负面影响。
