Food and Feed Immunology Group, Laboratory of Animal Food Function, Graduate School of Agricultural Science, Tohoku University, Sendai, Japan.
Livestock Immunology Unit, International Education and Research Center for Food and Agricultural Immunology (CFAI), Graduate School of Agricultural Science, Tohoku University, Sendai, Japan.
Front Immunol. 2023 Jul 3;14:1117102. doi: 10.3389/fimmu.2023.1117102. eCollection 2023.
culture models that precisely mirror the porcine respiratory epithelium are needed to gain insight into how pathogens and host interact. In this study, a new porcine bronchial epithelial cell line, designated as PBE cells, was established from the respiratory tract of a neonatal pig. PBE cells assumed a cobblestone-epithelial like morphology with close contacts between the cells when they reached confluence. The PBE cell line was characterized in terms of its expression of pattern recognition receptors (PRRs) and its ability to respond to the activation of the Toll-like receptor 3 (TLR3) and TLR4 signaling pathways, which are key PRRs involved in the defense of the respiratory epithelium against pathogens. PBE cells stimulated with poly(I:C) were able to up-regulate the expression of , (), (), the antiviral factors , , and , as well as the viral PRRs and The expression kinetics studies of immune factors in PBE cells allow us to speculate that this cell line can be a useful tool to investigate treatments that help to potentiate antiviral immunity in the respiratory epithelium of the porcine host. In addition, poly(I:C) and LPS treatments increased the expression of the inflammatory cytokines , and and differentially modulated the expression of negative regulators of the TLR signaling pathways. Then, PBE cells may also allow the evaluation of treatments that can regulate TLR3- and TLR4-mediated inflammatory injury in the porcine airway, thereby protecting the host against harmful overresponses.
需要能够精确模拟猪呼吸道上皮细胞的培养模型，以便深入了解病原体和宿主的相互作用方式。本研究中，我们从新生仔猪的呼吸道中建立了一种新的猪支气管上皮细胞系，命名为 PBE 细胞。当 PBE 细胞达到汇合状态时，其呈现鹅卵石样上皮细胞形态，细胞间紧密连接。我们对 PBE 细胞系进行了鉴定，包括其模式识别受体（PRRs）的表达情况以及对 Toll 样受体 3（TLR3）和 TLR4 信号通路激活的反应能力，这些 PRRs 是防御呼吸道上皮细胞免受病原体侵害的关键 PRRs。用 Poly(I:C)刺激 PBE 细胞能够上调 、 、 、抗病毒因子 、 、 和 以及病毒 PRRs 和 的表达。对 PBE 细胞中免疫因子表达的动力学研究使我们推测，该细胞系可以成为一种有用的工具，用于研究有助于增强猪宿主呼吸道上皮抗病毒免疫的治疗方法。此外，Poly(I:C)和 LPS 处理还增加了炎症细胞因子 和 的表达，并对 TLR 信号通路的负调控因子的表达进行了差异调节。因此，PBE 细胞还可以用于评估能够调节 TLR3 和 TLR4 介导的猪气道炎症损伤的治疗方法，从而保护宿主免受有害的过度反应。
