Zhang Shanyong, Ding Lei, Chen Gaoyang, Zhang Jiayin, Ge Wanbao, Qu Yuan
Department of Spine Surgery, The Second Hospital of Jilin University, Changchun, China.
Department of Rehabilitation, The Second Hospital of Jilin University, Changchun, China.
Front Bioeng Biotechnol. 2023 Jul 3;11:1209752. doi: 10.3389/fbioe.2023.1209752. eCollection 2023.
Introducing bone regeneration-promoting factors into scaffold materials to improve the bone induction property is crucial in the fields of bone tissue engineering and regenerative medicine. This study aimed to develop a Sr-HA/PTH-loaded composite hydrogel system with high biocompatibility. Teriparatide (PTH) capable of promoting bone regeneration was selected as the bioactive factor. Strontium-substituted hydroxyapatite (Sr-HA) was introduced into the system to absorb PTH to promote the bioactivity and delay the release cycle. PTH-loaded Sr-HA was then mixed with the precursor solution of the hydrogel to prepare the composite hydrogel as bone-repairing material with good biocompatibility and high mechanical strength. The experiments showed that Sr-HA absorbed PTH and achieved the slow and effective release of PTH. biological experiments showed that the Sr-HA/PTH-loaded hydrogel system had high biocompatibility, allowing the good growth of cells on the surface. The measurement of alkaline phosphatase activity and osteogenesis gene expression demonstrated that this composite system could promote the differentiation of MC3T3-E1 cells into osteoblasts. In addition, the cranial bone defect repair experiment confirmed that this composite hydrogel could promote the regeneration of new bones. In summary, Sr-HA/PTH composite hydrogel is a highly promising bone repair material.
将促进骨再生的因子引入支架材料以改善骨诱导性能在骨组织工程和再生医学领域至关重要。本研究旨在开发一种具有高生物相容性的载锶羟基磷灰石/甲状旁腺激素复合水凝胶系统。选择能够促进骨再生的特立帕肽（PTH）作为生物活性因子。将锶取代的羟基磷灰石（Sr-HA）引入该系统以吸附PTH，从而促进生物活性并延缓释放周期。然后将载有PTH的Sr-HA与水凝胶的前体溶液混合，制备具有良好生物相容性和高机械强度的复合水凝胶作为骨修复材料。实验表明，Sr-HA吸附了PTH并实现了PTH的缓慢有效释放。生物学实验表明，载Sr-HA/PTH的水凝胶系统具有高生物相容性，允许细胞在其表面良好生长。碱性磷酸酶活性和骨生成基因表达的测量表明，该复合系统可以促进MC3T3-E1细胞向成骨细胞分化。此外，颅骨缺损修复实验证实，这种复合水凝胶可以促进新骨的再生。综上所述，Sr-HA/PTH复合水凝胶是一种极具前景的骨修复材料。
