基于唑类/抗生素相互作用的改进方法，用于估算唑类抗真菌药对念珠菌属的抑制浓度。

Improved method for estimation of azole antifungal inhibitory concentrations against Candida species, based on azole/antibiotic interactions.

作者信息

Odds F C, Abbott A B, Pye G, Troke P F

出版信息

J Med Vet Mycol. 1986 Aug;24(4):305-11. doi: 10.1080/02681218680000461.

Abstract

Low, reproducible minimal inhibitory concentrations against Candida species, with sharp, precise end points in complex media were achieved for imidazoles (clotrimazole, econazole, miconazole, tioconazole and ketoconazole) and triazoles (fluconazole, itraconazole, vibunazole, ICI 153066) by including in the test medium antibacterial antibiotics that bind to the 80S eukaryotic ribosome and inhibit protein synthesis, i.e. blasticidin, cycloheximide, doxycycline, neomycin and gentamicin. The presence of these antibiotics reduced MICs, on average, by 50- to 250-fold. Other protein synthesis inhibitors (rifampicin, erythromycin, lincomycin, clindamycin, chloramphenicol and fusidic acid) were not effective, and the antibiotics did not affect MICs for Aspergillus species. The low azole MICs were in close agreement with MICs obtained in a defined, tissue culture-based medium lacking added antibiotics.

摘要

通过在测试培养基中加入能与80S真核核糖体结合并抑制蛋白质合成的抗菌抗生素，即杀稻瘟菌素、放线菌酮、强力霉素、新霉素和庆大霉素，咪唑类药物（克霉唑、益康唑、咪康唑、噻康唑和酮康唑）和三唑类药物（氟康唑、伊曲康唑、维布康唑、ICI 153066）对念珠菌属实现了低的、可重复的最低抑菌浓度，在复合培养基中有清晰、精确的终点。这些抗生素的存在使最低抑菌浓度平均降低了50至250倍。其他蛋白质合成抑制剂（利福平、红霉素、林可霉素、克林霉素、氯霉素和夫西地酸）无效，且这些抗生素不影响曲霉属的最低抑菌浓度。低的唑类最低抑菌浓度与在缺乏添加抗生素的特定组织培养基中获得的最低抑菌浓度非常一致。