在三种培养基中对新型隐球菌进行药敏试验时肉汤微量稀释法和半固体琼脂稀释法的双位点比较
Two-site comparison of broth microdilution and semisolid agar dilution methods for susceptibility testing of Cryptococcus neoformans in three media.
作者信息
Anaissie E, Shawar R, Paetznick V, Ensign L G, Witte Z, LaRocco M
机构信息
Department of Infectious Diseases, University of Texas M.D. Anderson Cancer Center, Houston 77030.
出版信息
J Clin Microbiol. 1993 May;31(5):1370-2. doi: 10.1128/jcm.31.5.1370-1372.1993.
Abstract
This study evaluated the inter- and intralaboratory agreement between results of the semisolid agar dilution and broth microdilution methods of antifungal susceptibility testing of Cryptococcus neoformans. Three media were tested in two laboratories. The drugs tested were amphotericin B, flucytosine, itraconazole, fluconazole, and Schering 39304. Analysis by kappa statistics revealed good agreement between the laboratories for the two methods. The highest level of inter- and intralaboratory agreement was observed in RPMI 1640 with L-glutamine followed by Eagle's minimum essential medium and yeast nitrogen broth. The broth microdilution method appears more suitable than the semisolid agar dilution method for testing cryptococci because of its ease in performance, cost, and simplicity.
摘要
本研究评估了新型隐球菌抗真菌药敏试验的半固体琼脂稀释法和肉汤微量稀释法结果在实验室间及实验室内部的一致性。在两个实验室中对三种培养基进行了测试。所检测的药物为两性霉素B、氟胞嘧啶、伊曲康唑、氟康唑和先灵葆雅39304。通过kappa统计分析显示，两种方法在实验室间具有良好的一致性。在添加L-谷氨酰胺的RPMI 1640培养基中观察到最高水平的实验室间及实验室内部一致性，其次是伊格尔最低基本培养基和酵母氮肉汤。由于肉汤微量稀释法操作简便、成本低且简单，因此对于检测隐球菌而言，该方法似乎比半固体琼脂稀释法更合适。
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Two-site comparison of broth microdilution and semisolid agar dilution methods for susceptibility testing of Cryptococcus neoformans in three media.在三种培养基中对新型隐球菌进行药敏试验时肉汤微量稀释法和半固体琼脂稀释法的双位点比较
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