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Two-site comparison of broth microdilution and semisolid agar dilution methods for susceptibility testing of Cryptococcus neoformans in three media.在三种培养基中对新型隐球菌进行药敏试验时肉汤微量稀释法和半固体琼脂稀释法的双位点比较
J Clin Microbiol. 1993 May;31(5):1370-2. doi: 10.1128/jcm.31.5.1370-1372.1993.
2
Collaborative investigation of broth microdilution and semisolid agar dilution for in vitro susceptibility testing of Candida albicans.肉汤微量稀释法和半固体琼脂稀释法用于白色念珠菌体外药敏试验的协同研究
J Clin Microbiol. 1992 Aug;30(8):1976-81. doi: 10.1128/jcm.30.8.1976-1981.1992.
3
Comparison of broth dilution and semisolid agar dilution for in vitro susceptibility testing of Cryptococcus neoformans.新型隐球菌体外药敏试验中肉汤稀释法与半固体琼脂稀释法的比较。
J Chemother. 1994 Jun;6(3):173-6. doi: 10.1080/1120009x.1994.11741148.
4
Comparison of the Etest and microdilution method for antifungal susceptibility testing of Cryptococcus neoformans to four antifungal agents.新型隐球菌对四种抗真菌药物的抗真菌药敏试验中Etest法与微量稀释法的比较。
J Antimicrob Chemother. 2000 Dec;46(6):997-1000. doi: 10.1093/jac/46.6.997.
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Multi-centre evaluation of the Etest method for antifungal drug susceptibility testing of Candida spp. and Cryptococcus neoformans. BSAC Working Party on Antifungal Chemotherapy.念珠菌属和新型隐球菌抗真菌药敏试验的Etest法多中心评估。英国抗菌化疗学会工作组
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Susceptibility testing of Cryptococcus neoformans: a microdilution technique.新型隐球菌药敏试验：一种微量稀释技术。
J Clin Microbiol. 1992 Nov;30(11):2881-6. doi: 10.1128/jcm.30.11.2881-2886.1992.
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Multicenter evaluation of broth microdilution method for susceptibility testing of Cryptococcus neoformans against fluconazole.新型隐球菌对氟康唑药敏试验肉汤微量稀释法的多中心评估
J Clin Microbiol. 1996 May;34(5):1280-2. doi: 10.1128/jcm.34.5.1280-1282.1996.
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Effect of media on growth rate and susceptibility testing of Cryptococcus neoformans.培养基对新型隐球菌生长速率及药敏试验的影响
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In vitro susceptibility of Cryptococcus neoformans clinical isolates from Egypt to seven antifungal drugs.埃及新型隐球菌临床分离株对七种抗真菌药物的体外敏感性
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Trends in antifungal drug susceptibility of Cryptococcus neoformans isolates in the United States: 1992 to 1994 and 1996 to 1998.美国新型隐球菌分离株对抗真菌药物的敏感性趋势：1992年至1994年及1996年至1998年。
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Correlation of fluconazole MICs with clinical outcome in cryptococcal infection.氟康唑最低抑菌浓度与隐球菌感染临床结局的相关性。
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Detection of resistance to amphotericin B among Cryptococcus neoformans clinical isolates: performances of three different media assessed by using E-test and National Committee for Clinical Laboratory Standards M27-A methodologies.新型隐球菌临床分离株中两性霉素B耐药性的检测：采用E-test和美国国家临床实验室标准委员会M27-A方法评估三种不同培养基的性能
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A vitamin-free minimal synthetic medium for Cryptococcus neoformans.一种用于新型隐球菌的无维生素基本合成培养基。
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7
In vitro susceptibilities of clinical and environmental isolates of Cryptococcus neoformans to five antifungal drugs.新型隐球菌临床分离株和环境分离株对五种抗真菌药物的体外敏感性
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本文引用的文献

1
Analysis of pH and buffer effects on flucytosine activity in broth dilution susceptibility testing of Candida albicans in two synthetic media.在两种合成培养基中对白色念珠菌进行肉汤稀释药敏试验时，pH值和缓冲液对氟胞嘧啶活性影响的分析
Antimicrob Agents Chemother. 1984 Sep;26(3):364-7. doi: 10.1128/AAC.26.3.364.
2
Cryptococcemia.隐球菌血症
Medicine (Baltimore). 1983 Mar;62(2):98-109. doi: 10.1097/00005792-198303000-00003.
3
Fungemia in a cancer hospital: changing frequency, earlier onset, and results of therapy.一家癌症医院的真菌血症：发生率的变化、发病时间的提前及治疗结果
Rev Infect Dis. 1985 Sep-Oct;7(5):646-55. doi: 10.1093/clinids/7.5.646.
4
Microtiter broth dilution method for yeast susceptibility testing with validation by clinical outcome.采用临床结果验证的微量滴定肉汤稀释法进行酵母药敏试验。
J Clin Microbiol. 1986 Oct;24(4):600-6. doi: 10.1128/jcm.24.4.600-606.1986.
5
Improved method for estimation of azole antifungal inhibitory concentrations against Candida species, based on azole/antibiotic interactions.基于唑类/抗生素相互作用的改进方法，用于估算唑类抗真菌药对念珠菌属的抑制浓度。
J Med Vet Mycol. 1986 Aug;24(4):305-11. doi: 10.1080/02681218680000461.
6
Comparison of relative susceptibilities of Candida species to three antifungal agents as determined by unstandardized methods.用非标准化方法测定念珠菌属对三种抗真菌药物的相对敏感性比较。
Antimicrob Agents Chemother. 1987 Sep;31(9):1343-7. doi: 10.1128/AAC.31.9.1343.
7
Influence of culture medium on susceptibility testing with BAY n 7133 and ketoconazole.培养基对BAY n 7133和酮康唑药敏试验的影响。
J Clin Microbiol. 1986 Aug;24(2):269-71. doi: 10.1128/jcm.24.2.269-271.1986.
8
Results of a survey of antifungal susceptibility tests in the United States and interlaboratory comparison of broth dilution testing of flucytosine and amphotericin B.美国抗真菌药敏试验调查结果以及氟胞嘧啶和两性霉素B肉汤稀释试验的实验室间比较。
J Clin Microbiol. 1986 Feb;23(2):298-301. doi: 10.1128/jcm.23.2.298-301.1986.
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Antifungal susceptibility tests.抗真菌药敏试验。
Antimicrob Agents Chemother. 1987 Dec;31(12):1867-70. doi: 10.1128/AAC.31.12.1867.
10
Cryptococcal meningitis in patients with AIDS.艾滋病患者的隐球菌性脑膜炎
J Infect Dis. 1988 Apr;157(4):624-8. doi: 10.1093/infdis/157.4.624.

在三种培养基中对新型隐球菌进行药敏试验时肉汤微量稀释法和半固体琼脂稀释法的双位点比较

Two-site comparison of broth microdilution and semisolid agar dilution methods for susceptibility testing of Cryptococcus neoformans in three media.

作者信息

Anaissie E, Shawar R, Paetznick V, Ensign L G, Witte Z, LaRocco M

机构信息

Department of Infectious Diseases, University of Texas M.D. Anderson Cancer Center, Houston 77030.

出版信息

J Clin Microbiol. 1993 May;31(5):1370-2. doi: 10.1128/jcm.31.5.1370-1372.1993.

DOI:10.1128/jcm.31.5.1370-1372.1993
PMID:8501245
原文链接:https://pmc.ncbi.nlm.nih.gov/articles/PMC262942/
Abstract

This study evaluated the inter- and intralaboratory agreement between results of the semisolid agar dilution and broth microdilution methods of antifungal susceptibility testing of Cryptococcus neoformans. Three media were tested in two laboratories. The drugs tested were amphotericin B, flucytosine, itraconazole, fluconazole, and Schering 39304. Analysis by kappa statistics revealed good agreement between the laboratories for the two methods. The highest level of inter- and intralaboratory agreement was observed in RPMI 1640 with L-glutamine followed by Eagle's minimum essential medium and yeast nitrogen broth. The broth microdilution method appears more suitable than the semisolid agar dilution method for testing cryptococci because of its ease in performance, cost, and simplicity.

摘要

本研究评估了新型隐球菌抗真菌药敏试验的半固体琼脂稀释法和肉汤微量稀释法结果在实验室间及实验室内部的一致性。在两个实验室中对三种培养基进行了测试。所检测的药物为两性霉素B、氟胞嘧啶、伊曲康唑、氟康唑和先灵葆雅39304。通过kappa统计分析显示，两种方法在实验室间具有良好的一致性。在添加L-谷氨酰胺的RPMI 1640培养基中观察到最高水平的实验室间及实验室内部一致性，其次是伊格尔最低基本培养基和酵母氮肉汤。由于肉汤微量稀释法操作简便、成本低且简单，因此对于检测隐球菌而言，该方法似乎比半固体琼脂稀释法更合适。