Zhou Zhanmei, Fan Jiangbo, Zhang Jia, Yang Yanmei, Zhang Yifan, Zan Xiaofei, Li Xiaohong, Wan Jiale, Gao Xiaoling, Chen Rongjun, Huang Zhengjian, Xu Zhengjun, Li Lihua
Crop Ecophysiology and Cultivation Key Laboratory of Sichuan Province, Rice Research Institute of Sichuan Agricultural University, Chengdu 611130, China.
Chongqing Army Characteristic Medical Center, Chongqing 400000, China.
Plants (Basel). 2022 Jun 23;11(13):1653. doi: 10.3390/plants11131653.
Rice ( L.) is one of the main food crops for human survival, and its yield is often restricted by abiotic stresses. Drought and soil salinity are among the most damaging abiotic stresses affecting today's agriculture. Given the importance of abscisic acid (ABA) in plant growth and abiotic stress responses, it is very important to identify new genes involved in ABA signal transduction. We screened a drought-inducing gene containing about 158 amino acid residues from the transcriptome library of rice exposed to drought treatment, and we found ABA-related cis-acting elements and multiple drought-stress-related cis-acting elements in its promoter sequence. The results of real-time PCR showed that was strongly induced by drought and salt stresses. The physiological and biochemical phenotype analysis of transgenic plants confirmed that overexpression of enhanced the tolerance to drought and salt stresses in rice. The expression of -GFP fusion protein indicated that was located in both the cell membrane system and nucleus. Compared with the wild type, the overexpressed showed enhanced sensitivity to ABA. Physiological analyses showed that the overexpression of may regulate the water loss efficiency and ABA-responsive gene expression of rice plants under drought and salt stresses, and it reduces membrane damage and the accumulation of reactive oxygen species. These results indicate that is a positive regulator of drought and salinity tolerance in rice, governing the tolerance of rice to abiotic stresses through an ABA-dependent pathway. Therefore, this study provides a new insight into the physiological and molecular mechanisms of -mediated ABA signal transduction participating in drought and salt stresses.
水稻（Oryza sativa L.）是人类赖以生存的主要粮食作物之一，其产量常受非生物胁迫的限制。干旱和土壤盐渍化是影响当今农业的最具破坏性的非生物胁迫因素。鉴于脱落酸（ABA）在植物生长和非生物胁迫响应中的重要性，鉴定参与ABA信号转导的新基因非常重要。我们从经干旱处理的水稻转录组文库中筛选出一个含有约158个氨基酸残基的干旱诱导基因，并且在其启动子序列中发现了ABA相关的顺式作用元件和多个干旱胁迫相关的顺式作用元件。实时PCR结果表明，该基因受干旱和盐胁迫强烈诱导。转基因植物的生理生化表型分析证实，该基因的过表达增强了水稻对干旱和盐胁迫的耐受性。该基因与绿色荧光蛋白（GFP）融合蛋白的表达表明，该基因定位于细胞膜系统和细胞核中。与野生型相比，过表达该基因的植株对ABA表现出增强的敏感性。生理分析表明，该基因的过表达可能调控干旱和盐胁迫下水稻植株的水分散失效率和ABA响应基因的表达，并减少膜损伤和活性氧的积累。这些结果表明，该基因是水稻干旱和盐耐受性的正调控因子，通过依赖ABA的途径调控水稻对非生物胁迫的耐受性。因此，本研究为该基因介导的ABA信号转导参与干旱和盐胁迫的生理和分子机制提供了新的见解。
