Comparative analysis of mitochondrial genomes of and .

The family Schisandraceae is a basal angiosperm plant group distributed in East and Southeast Asia and includes many medicinal plant species such as . In this study, mitochondrial genomes (mitogenomes) of two species, and , in the family were characterized through assembly using sequencing data obtained with Oxford Nanopore and Illumina sequencing technologies. The mitogenomes of were assembled into one circular contig (571,107 bp) and four linear contigs (10,898-607,430 bp), with a total of 60 genes: 38 protein-coding genes (PCGs), 19 tRNA genes, and 3 rRNA genes. The mitogenomes of were assembled into five circular contigs (211,474-973,503 bp) and three linear contigs (8,010-72,712 bp), with a total of 66 genes: 44 PCGs, 19 tRNA genes, and 3 rRNA genes. The mitogenomes of the two species had complex structural features with high repeat numbers and chloroplast-derived sequences, as observed in other plant mitogenomes. Phylogenetic analysis based on PCGs revealed the taxonomical relationships of and with other species from Schisandraceae. Finally, molecular markers were developed to distinguish between , , and on the basis of InDel polymorphisms present in the mitogenomes. The mitogenomes of and will be valuable resources for molecular and taxonomic studies of plant species that belong to the family Schisandraceae.