Ashby J, Mohammed R
Mutat Res. 1986 Aug;164(4):217-35. doi: 10.1016/0165-1161(86)90056-7.
This paper describes the results of a study in which mouse bone marrow micronucleus assay slides were assessed for homogeneity of micronucleated polychromatic erythrocytes (MPE) among polychromatic erythrocytes (PE). The slides were prepared by 3 distinct methods and several methods of slide reading were assessed. Observations made using our slides were confirmed by re-analysis of slides from 3 independent laboratories. It is concluded that the method of slide preparation and assessment can significantly influence the variability of data obtained from a study. The extent of this variability casts doubt upon the validity of certain assumptions concerning this assay--such as sex differences in MPE incidence, responder variability, etc. Results are discussed within the context of the very recent literature for this assay. Some laboratories appear to have adequate methods of slide preparation and data accumulation, while others do not. Methods to improve the sensitivity of this assay are suggested within the context of the recommendations made by the Gene-Tox review group. In particular, it is suggested that individual investigators present evidence of the adequacy of their data accumulation techniques in order to enhance the value of future studies.
本文描述了一项研究的结果，该研究对小鼠骨髓微核试验玻片进行评估，以检测多染性红细胞（PE）中微核多染性红细胞（MPE）的同质性。玻片通过3种不同方法制备，并评估了几种玻片读数方法。通过对来自3个独立实验室的玻片进行重新分析，证实了使用我们的玻片所做的观察结果。得出的结论是，玻片制备和评估方法可显著影响研究所得数据的变异性。这种变异性的程度对有关该试验的某些假设的有效性提出了质疑，例如MPE发生率的性别差异、反应者变异性等。在该试验的最新文献背景下讨论了结果。一些实验室似乎有足够的玻片制备和数据积累方法，而其他实验室则没有。在基因毒性审查小组提出的建议背景下，提出了提高该试验灵敏度的方法。特别是，建议个体研究者提供其数据积累技术充分性的证据，以提高未来研究的价值。
