Collaborative Innovation Center of Henan Province for Green Manufacturing of Fine Chemicals, Key Laboratory of Green Chemical Media and Reactions, Ministry of Education, School of Chemistry and Chemical Engineering, Henan Normal University, Xinxiang, Henan 453007, P. R. China.
Institute of Pediatrics, Shenzhen Children's Hospital, Shenzhen 518038, Guangdong, P. R. China.
J Mater Chem B. 2023 Sep 6;11(32):7675-7683. doi: 10.1039/d3tb00957b.
Bacterial infections have been a major threat to human health. Especially, Gram-negative (G) bacterial infections have been an increasing problem worldwide. The overuse of antibiotics leads to an emergence of drug resistance, and thus the development of novel antimicrobial agents is important, particularly against G bacteria. Nanozymes use reactive oxygen species (ROS) to kill bacteria, reducing the risk of bacterial resistance and providing new opportunities to meet the challenges of strain selectivity. Here, we synthesized NiCo layered double hydroxide (LDH) nanozymes, which exhibit selective antibacterial activity based on their peroxide-like (POD-like) activity. To obtain the highest antibacterial activity, the POD-like activity of NiCo LDH nanozyme was further optimized by tuning the ratio of nickel and cobalt, and NiCo LDHs showed the highest POD activity and antibacterial activity. More importantly, NiCo LDHs can achieve selective sterilization of G bacteria due to their electrostatic adsorption and hydrophilic interactions with the bacterial cell wall. Animal experiments further indicated that the healing of G bacteria-infected wounds was effectively promoted without damaging their normal biological tissues. In conclusion, we provide a selective antibacterial agent through a simple strategy, which provides a new direction for the application of nanozymes.
细菌感染一直是人类健康的主要威胁。特别是，革兰氏阴性（G）细菌感染已成为全球日益严重的问题。抗生素的过度使用导致了耐药性的出现，因此开发新型抗菌剂非常重要，尤其是针对 G 细菌。纳米酶利用活性氧（ROS）杀死细菌，降低了细菌耐药性的风险，并为应对菌株选择性的挑战提供了新的机会。在这里，我们合成了 NiCo 层状双氢氧化物（LDH）纳米酶，其基于过氧化物酶样（POD-like）活性表现出选择性的抗菌活性。为了获得最高的抗菌活性，我们通过调整镍和钴的比例进一步优化了 NiCo LDH 纳米酶的 POD-like 活性，NiCo LDH 表现出最高的 POD 活性和抗菌活性。更重要的是，NiCo LDH 可以通过与细菌细胞壁的静电吸附和亲水性相互作用实现对 G 细菌的选择性杀菌。动物实验进一步表明，NiCo LDH 可以有效地促进 G 细菌感染伤口的愈合，而不会损伤其正常的生物组织。总之，我们通过一种简单的策略提供了一种选择性的抗菌剂，为纳米酶的应用提供了新的方向。
