Plant Metabolism and Nutrition Laboratory, Faculty of Agricultural Science, Federal University of Grande Dourados, Dourados, MS, Brazil.
Biotecland Tecnologias Agrícolas LTDA, Jataí, GO, Brazil.
BMC Plant Biol. 2024 Nov 29;24(1):1149. doi: 10.1186/s12870-024-05646-9.
Seed conditioning with bioinputs (biopriming) offers a promising and sustainable alternative to mitigate the adverse effects of salt stress on soybeans. This study aims to evaluate the potential of isolated or combined biopriming using microalgae and different microorganisms in alleviating salinity-induced stress in soybeans in early-stage.
Seeds were subjected to five biopriming treatments: Parachlorella sp. microalgae culture, Bacillus subtilis, Trichoderma harzianum, Parachlorella sp. + B. subtilis, and Parachlorella sp. + T. harzianum, along with a control group without biopriming. Subsequently, the seeds were exposed to two conditions: (i) control (0.0 MPa), and (ii) salinity induced by NaCl (-0.8 MPa). Germination, photochemical indicators, and seedling performance were assessed.
Salinity impaired root protrusion and seed physiology, resulting in a high percentage of abnormal seedlings, thus creating a stressful condition. However, biopriming alleviated the negative effects of salinity, particularly with T. harzianum, Parachlorella sp. + B. subtilis, and Parachlorella sp. + T. harzianum, which led to high germination rates and normal seedlings. All biopriming treatments, especially the combined ones, reduced the suppression of non-photochemical quenching, thereby enhancing the maximum yield of photosystem II. Seedlings under salt stress without biopriming exhibited short lengths and low fresh and dry mass, whereas those bioprimed with Parachlorella sp. + B. subtilis and Parachlorella sp. + T. harzianum showed significantly higher values.
Seed biopriming, especially with Parachlorella sp. microalgae culture combined with B. subtilis or T. harzianum, effectively alleviates the stressful effects of salinity on germination and early-stage growth seedling of soybeans.
用生物投入物（生物引发）对种子进行处理为缓解大豆盐胁迫的不良影响提供了一种有前途且可持续的替代方法。本研究旨在评估单独或组合使用微藻和不同微生物进行生物引发以缓解早期大豆盐胁迫的潜力。
种子接受了五种生物引发处理：小球藻微藻培养物、枯草芽孢杆菌、哈茨木霉、小球藻+枯草芽孢杆菌和小球藻+哈茨木霉，以及未进行生物引发的对照组。随后，种子暴露于两种条件下：（i）对照（0.0 MPa）和（ii）由 NaCl 诱导的盐度（-0.8 MPa）。评估发芽率、光化学指标和幼苗性能。
盐度损害了根的突出和种子的生理机能，导致高比例的异常幼苗，从而形成了一种胁迫环境。然而，生物引发减轻了盐度的负面影响，特别是哈茨木霉、小球藻+枯草芽孢杆菌和小球藻+哈茨木霉，它们导致高发芽率和正常幼苗。所有生物引发处理，特别是组合处理，减少了非光化学猝灭的抑制，从而提高了光合作用系统 II 的最大产量。未进行生物引发的盐胁迫下的幼苗表现出较短的长度和较低的鲜重和干重，而用小球藻+枯草芽孢杆菌和小球藻+哈茨木霉进行生物引发的幼苗则表现出显著更高的值。
种子生物引发，特别是小球藻微藻培养物与枯草芽孢杆菌或哈茨木霉的组合，可有效缓解盐胁迫对大豆发芽和早期生长幼苗的胁迫影响。
