Department of Veterinary and Animal Sciences, University of Copenhagen, Frederiksberg, Denmark.
Department of Veterinary Clinical Sciences, University of Copenhagen, Frederiksberg, Denmark.
J Vet Intern Med. 2023 Sep-Oct;37(5):1738-1749. doi: 10.1111/jvim.16813. Epub 2023 Jul 24.
Differentiation of gastrointestinal cancer (GIC) from chronic inflammatory enteropathies (CIE) in cats can be challenging and often requires extensive diagnostic testing. MicroRNAs (miRNAs) have promise as non-invasive biomarkers in serum and feces for diagnosis of GIC.
HYPOTHESIS/OBJECTIVES: Cats with GIC will have serum and fecal miRNA profiles that differ significantly from healthy cats and cats with CIE. Identify serum and fecal miRNAs with diagnostic potential for differentiation between cats with GIC and CIE as compared to healthy cats.
Ten healthy cats, 9 cats with CIE, and 10 cats with GIC; all client-owned.
Cats were recruited for an international multicenter observational prospective case-control study. Serum and feces were screened using small RNA sequencing for miRNAs that differed in abundance between cats with GIC and CIE, and healthy cats. Diagnostic biomarker potential of relevant miRNAs from small RNA sequencing and the literature was confirmed using reverse transcription quantitative real-time PCR (RT-qPCR).
Serum miR-223-3p was found to distinguish between cats with GIC and CIE with an area under the curve (AUC) of 0.9 (95% confidence interval [CI], 0.760-1.0), sensitivity of 90% (95% CI, 59.6-99.5%), and specificity of 77.8% (95% CI, 45.3-96.1%). Serum miR-223-3p likewise showed promise in differentiating a subgroup of cats with small cell lymphoma (SCL) from those with CIE. No fecal miRNAs could distinguish between cats with GIC and CIE.
Serum miR-223-3p potentially may serve as a noninvasive diagnostic biomarker of GIC in cats, in addition to providing a much needed tool for the differentiation of CIE and SCL.
猫的胃肠道癌症（GIC）与慢性炎症性肠病（CIE）的区分具有挑战性，通常需要广泛的诊断测试。microRNAs（miRNAs）作为血清和粪便中用于诊断 GIC 的非侵入性生物标志物具有一定的前景。
假设/目的：患有 GIC 的猫的血清和粪便 miRNA 图谱与健康猫和患有 CIE 的猫有显著差异。与健康猫相比，确定用于区分患有 GIC 和 CIE 的猫的血清和粪便中具有诊断潜力的 miRNAs。
10 只健康猫、9 只患有 CIE 的猫和 10 只患有 GIC 的猫；均为客户所有。
招募猫进行国际多中心观察性前瞻性病例对照研究。使用小 RNA 测序筛选血清和粪便中的 miRNAs，这些 miRNAs 在 GIC 和 CIE 猫与健康猫之间的丰度存在差异。使用逆转录定量实时 PCR（RT-qPCR）对小 RNA 测序和文献中相关 miRNAs 的诊断生物标志物潜力进行确认。
发现血清 miR-223-3p 可区分 GIC 和 CIE 猫，曲线下面积（AUC）为 0.9（95%置信区间[CI]，0.760-1.0），敏感性为 90%（95%CI，59.6-99.5%），特异性为 77.8%（95%CI，45.3-96.1%）。血清 miR-223-3p 同样有望区分小细胞淋巴瘤（SCL）和 CIE 猫。没有粪便 miRNAs 可以区分 GIC 和 CIE 猫。
血清 miR-223-3p 可能是猫 GIC 的非侵入性诊断生物标志物，此外还为区分 CIE 和 SCL 提供了急需的工具。
