State Key Laboratory of Microbial Metabolism, School of Life Sciences and Biotechnology, Shanghai Jiao Tong University, Shanghai, 200240, PR China.
School of Physics and Astronomy, Shanghai Jiao Tong University, Shanghai, 200240, PR China.
Int J Syst Evol Microbiol. 2023 Jul;73(7). doi: 10.1099/ijsem.0.005972.
A novel member of class was isolated from marine sediment of the South China Sea. Cells of strain LMO-2 were Gram-stain negative, greyish in colour, motile, with a single lateral flagellum and short rod in shape with a slight curve. Strain LMO-2 was positive for oxidase and negative for catalase. The bacterium grew aerobically at 10-40 °C (optimum, 25-30 °C), pH 5.5-10.0 (optimum, pH 7.0) and 0-9 % NaCl (w/v; optimum, 2-3 %). Phylogenetic analysis of the 16S rRNA gene sequence and phylogenomic analysis of the whole genome sequence indicated that strain LMO-2 represents a new genus and a new species within the family , class , phylum . Comparisons of the 16S rRNA gene sequences of strain LMO-2 showed 94.8 % similarity to its closest relative. The genome size is ~3.45 Mbp with a DNA G+C content of 58.17 mol%. The strain possesses potential capability for the degradation of complex organic matter, i.e. fatty acid and benzoate. The predominant cellular fatty acids (>10 %) were C and C ω7c 11-methyl. The sole respiratory quinone was ubiquinone-10. The major identified polar lipids were diphosphatidylglycerol, phosphatidylglycerol and phospholipid. Based on the polyphasic taxonomic data, strain LMO-2 represents a novel genus and a novel species for which the name gen. nov., sp. nov., was proposed in the family . The type strain is LMO-2 (=CGMCC 1.19273=JCM 34934).
从南海海洋沉积物中分离到一株新型的。菌株 LMO-2 的细胞革兰氏染色阴性，呈灰色，可运动，具有单个侧鞭毛，呈短杆状，略带弯曲。菌株 LMO-2 氧化酶阳性，过氧化氢酶阴性。该菌需氧生长，最适生长温度为 10-40°C（最佳，25-30°C），pH 值为 5.5-10.0（最佳，pH 7.0），盐度为 0-9%NaCl（w/v；最佳，2-3%）。16S rRNA 基因序列的系统发育分析和全基因组序列的系统发育分析表明，菌株 LMO-2 代表了家族、纲、门中的一个新属和一个新种。菌株 LMO-2 的 16S rRNA 基因序列与最接近的亲缘关系相似度为 94.8%。基因组大小约为 3.45Mbp，GC 含量为 58.17%mol%。该菌株具有降解复杂有机物的潜在能力，例如脂肪酸和苯甲酸。主要的细胞脂肪酸（>10%）为 C 和 Cω7c 11-甲基。唯一的呼吸醌是泛醌-10。主要鉴定的极性脂类是双磷脂酰甘油、磷脂酰甘油和磷脂。基于多相分类学数据，菌株 LMO-2 代表了一个新属和一个新种，该属和种被提议为。模式菌株为 LMO-2（=CGMCC 1.19273=JCM 34934）。
