Instituto de Química, Universidade Federal de Goiás, 74690-900, Goiânia, GO, Brazil.
Instituto de Química, Universidade Federal de Uberlândia, 38408-100, Uberlândia, MG, Brazil; Instituto Nacional de Ciência e Tecnologia de Bioanalítica, 13084-971, Campinas, SP, Brazil.
Talanta. 2024 Jan 1;266(Pt 1):124960. doi: 10.1016/j.talanta.2023.124960. Epub 2023 Jul 17.
The number of cases in which scopolamine (SCO) was used for both recreational and predatory purposes has increased dramatically in recent decades. Linked to this, there is a concern about obtaining SCO through thermal degradation of butylscopolamine (BSCO) - an active ingredient of Buscopan® - a drug sold without a medical prescription. In this study, mixtures containing SCO and BSCO were separated and detected on a microchip electrophoresis (ME) device with integrated capacitively coupled contactless conductivity detection (CD) using a running buffer composed of 40 mmol L of butyric acid and 25 mmol L of sodium hydroxide (pH 5.0). The separation was performed within ca. 115 s with a resolution of 1.3 and separation efficiency ranging from 1.4 × 10 to 1.5 × 10 theoretical plates m. A detection limit of 1.1 μmol L was achieved for both species and the developed method revealed satisfactory repeatability with relative standard deviation (RSD) values for forty-eight injections between 4.8 and 9.4% for peak areas and lower than 3.3% for migration times. Furthermore, inter-day precision was evaluated for sixteen injections (a sequence of four injections performed over four days), and RSD values were less than 6.6% for peak areas and 2.2% for migration times. Satisfactory recovery values (95-114%) were obtained for all evaluated beverage samples (cachaça, vodka, whiskey, beer, Coca-Cola, and grape juice) as well as for artificial urine samples (95-107%). Finally, the conversion of BSCO into SCO was observed after simple heating procedure of Buscopan® sample (not subject to medical prescription), which was successfully confirmed through analysis by capillary electrophoresis coupled to the mass spectrometry (CE-MS). Based on the reported results, the use of ME-CD devices has demonstrated a huge potential for applications in the forensic chemistry field.
近年来，莨菪碱（SCO）被用于娱乐和掠夺性目的的案例数量急剧增加。与此相关的是，人们担心通过热降解丁基莨菪碱（BSCO）——一种无处方销售的药物 Buscopan®的有效成分——来获得 SCO。在这项研究中，使用含有 40mmol/L 丁酸和 25mmol/L 氢氧化钠（pH 5.0）的运行缓冲液，在带有集成电容耦合非接触式电导检测（CD）的微芯片电泳（ME）设备上分离和检测含有 SCO 和 BSCO 的混合物。分离在约 115s 内完成，分辨率为 1.3，分离效率在 1.4×10 到 1.5×10 理论板 m 之间。两种物质的检测限均达到 1.1μmol/L，所开发的方法显示出令人满意的重复性，48 次进样的峰面积和迁移时间的相对标准偏差（RSD）值分别为 4.8%至 9.4%和低于 3.3%。此外，对 16 次进样（在四天内进行四次进样的序列）进行了日间精密度评估，峰面积的 RSD 值小于 6.6%，迁移时间的 RSD 值小于 2.2%。所有评估的饮料样品（甘蔗酒、伏特加、威士忌、啤酒、可口可乐和葡萄汁）以及人工尿液样品（95-107%）均获得了令人满意的回收率（95-114%）。最后，在简单加热 Buscopan®样品（无需处方）后观察到 BSCO 转化为 SCO，这通过毛细管电泳与质谱（CE-MS）分析成功得到证实。基于报告的结果，ME-CD 设备在法医化学领域的应用具有巨大的潜力。
