School of Health Science and Engineering, University of Shanghai for Science and Technology, Shanghai 200093, China.
National Grain Industry (Urban Grain and Oil Security) Technology Innovation Center, Shanghai 200093, China.
Food Funct. 2023 Aug 14;14(16):7469-7477. doi: 10.1039/d3fo01951a.
Satiety hormone cholecystokinin (CCK) plays a vital role in appetite inhibition. Its secretion is regulated by dietary components. The search for bioactive compounds that stimulate CCK secretion is currently an active area of research. The objective of this study was to evaluate the ability of buckwheat ( Moench) protein digest (BPD) to stimulate CCK secretion and and clarify the structural characteristics of peptides stimulating CCK secretion. BPD was prepared by an gastrointestinal digestion model. The relative molecular weight of BPD was <10 000 Da, and peptides with <3000 Da accounted for 70%. BPD was rich in essential amino acids Lys, Leu, and Val but lacked sulfur amino acids Met and Cys. It had a stimulatory effect on CCK secretion and . Chromatographic separation was performed to isolate peptide fractions involved in CCK secretion, and five novel CCK-releasing peptides including QFDLDD, PAFKEEHL, SFHFPI, IPPLFP, and RVTVQPDS were successfully identified. A sequence length range of 6-8 and marked hydrophobicity (18-28) were observed among the most CCK-releasing peptides. The present study demonstrated for the first time that BPD could stimulate CCK secretion and clarify the structural characteristics of bioactive peptides having CCK secretagogue activity in BPD.
饱腹激素胆囊收缩素（CCK）在抑制食欲方面起着至关重要的作用。它的分泌受膳食成分的调节。目前，寻找能刺激 CCK 分泌的生物活性化合物是一个活跃的研究领域。本研究旨在评估荞麦（Moench）蛋白水解物（BPD）刺激 CCK 分泌的能力，并阐明刺激 CCK 分泌的肽的结构特征。BPD 是通过胃肠消化模型制备的。BPD 的相对分子质量<10000 Da，其中<3000 Da 的肽占 70%。BPD 富含必需氨基酸 Lys、Leu 和 Val，但缺乏含硫氨基酸 Met 和 Cys。它对 CCK 分泌有刺激作用。进行色谱分离以分离参与 CCK 分泌的肽级分，成功鉴定了五种新型 CCK 释放肽，包括 QFDLDD、PAFKEEHL、SFHFPI、IPPLFP 和 RVTVQPDS。最能释放 CCK 的肽中观察到序列长度范围为 6-8 个氨基酸且具有明显疏水性（18-28）。本研究首次证明 BPD 可以刺激 CCK 分泌，并阐明 BPD 中具有 CCK 促分泌活性的生物活性肽的结构特征。
