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[辐射暴露后血清及其成分的化学发光与抗性的比较研究]

[Comparative study of chemiluminescence and resistance of serum and its components after radiation exposure].

作者信息

Baraboĭ V A, Orel V E

出版信息

Radiobiologiia. 1986 Jul-Aug;26(4):547-53.

PMID:3749482
Abstract

Kinetics of spontaneous chemiluminescence (CL) and electrochemiluminescence (ECL) and resistance of blood serum and its protein, lipid and carbohydrate components under the effect of X-rays (3 to 1622 Gy) and the indirect effect of radiation initiated by the addition of hydrogen peroxide (1.5 X 10(-5)-1.5%) was studied to estimate the contribution of each of the serum components to cumulative changes in the kinetics of free radical oxidation initiated by the effect of radiation. There was a parametric dependence between the absorbed dose, the rate of ECL and the resistance of blood serum and its components. As the absorbed dose or hydrogen peroxide concentration increased ECL contribution to the cumulative luminescence signal regularly decreased. Changes in CL and ECL of blood serum induced by ionizing radiation and H2O2 were qualitatively similar. The kinetics of free radical oxidation of blood serum initiated by irradiation was determined integrally (according to CL and ECL parameters) by a complex of changes in its components.

摘要

研究了X射线(3至1622戈瑞)作用下以及添加过氧化氢(1.5×10⁻⁵ - 1.5%)引发的辐射间接作用下,血清及其蛋白质、脂质和碳水化合物成分的自发化学发光(CL)和电化学发光(ECL)动力学以及抗性,以评估血清各成分对辐射引发的自由基氧化动力学累积变化的贡献。吸收剂量、ECL速率与血清及其成分的抗性之间存在参数依赖性。随着吸收剂量或过氧化氢浓度的增加,ECL对累积发光信号的贡献有规律地降低。电离辐射和H₂O₂引起的血清CL和ECL变化在性质上相似。照射引发的血清自由基氧化动力学是由其成分的一系列变化整体决定的(根据CL和ECL参数)。

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