Welton Tarah A, George Nicholas M, Ozbay Baris N, Gentile Polese Arianna, Osborne Gregory, Futia Gregory L, Kushner J Keenan, Kleinschmidt-DeMasters Bette, Alexander Allyson L, Abosch Aviva, Ojemann Steven, Restrepo Diego, Gibson Emily A
Department of Bioengineering, University of Colorado Anschutz Medical Campus, Aurora, CO 80045, USA.
Neuroscience Graduate Program, University of Colorado Anschutz Medical Campus, Aurora, CO 80045, USA.
Biomed Opt Express. 2023 Jun 27;14(7):3705-3725. doi: 10.1364/BOE.492552. eCollection 2023 Jul 1.
We demonstrate a gradient refractive index (GRIN) microendoscope with an outer diameter of ∼1.2 mm and a length of ∼186 mm that can fit into a stereotactic surgical cannula. Two photon imaging at an excitation wavelength of 900 nm showed a field of view of ∼180 microns and a lateral and axial resolution of 0.86 microns and 9.6 microns respectively. The microendoscope was tested by imaging autofluorescence and second harmonic generation (SHG) in label-free human brain tissue. Furthermore, preliminary image analysis indicates that image classification models can predict if an image is from the subthalamic nucleus or the surrounding tissue using conventional, bench-top two-photon autofluorescence.
我们展示了一种梯度折射率（GRIN）微内窥镜，其外径约为1.2毫米，长度约为186毫米，可装入立体定向手术套管。在900纳米激发波长下的双光子成像显示视野约为180微米，横向分辨率和轴向分辨率分别为0.86微米和9.6微米。通过对无标记人脑组织中的自发荧光和二次谐波产生（SHG）进行成像，对该微内窥镜进行了测试。此外，初步图像分析表明，图像分类模型可以使用传统的台式双光子自发荧光预测图像是否来自丘脑底核或周围组织。
