de Wit Jos, Glentis George-Othon, Kalkman Jeroen
Department of Imaging Physics, Lorentzweg 1, 2628 CJ, Delft, The Netherlands.
Department of Informatics and Telecommunications, University of Peloponnese, Tripolis, 22100, Greece.
Biomed Opt Express. 2023 Jun 21;14(7):3532-3554. doi: 10.1364/BOE.487345. eCollection 2023 Jul 1.
Phase-preserving spectral estimation optical coherence tomography (SE-OCT) enables combining axial resolution improvement with computational depth of field (DOF) extension. We show that the combination of SE-OCT with interferometric synthetic aperture microscopy (ISAM) and computational adaptive optics (CAO) results in high 3D resolution over a large depth range for an OCT system with a narrow bandwidth visible light super-luminescent diode (SLD). SE-OCT results in up to five times axial resolution improvement from 8 µm to 1.5 µm. The combination with ISAM gives a sub-micron lateral resolution over a 400 µm axial range, which is at least 16 times the conventional depth of field. CAO can be successfully applied after SE and ISAM and removes residual aberrations, resulting in high quality images. The results show that phase-preserving SE-OCT is sufficiently accurate for coherent post-processing, enabling the use of cost-effective SLDs in the visible light range for high spatial resolution OCT.
保相光谱估计光学相干断层扫描（SE-OCT）能够将轴向分辨率的提高与计算景深（DOF）的扩展相结合。我们表明，将SE-OCT与干涉合成孔径显微镜（ISAM）和计算自适应光学（CAO）相结合，对于具有窄带宽可见光超发光二极管（SLD）的OCT系统，可在大深度范围内实现高3D分辨率。SE-OCT可使轴向分辨率从8 µm提高到1.5 µm，提高了多达五倍。与ISAM相结合，在400 µm轴向范围内可实现亚微米级横向分辨率，这至少是传统景深的16倍。CAO可在SE和ISAM之后成功应用，并消除残余像差，从而得到高质量图像。结果表明，保相SE-OCT对于相干后处理足够精确，能够在可见光范围内使用经济高效的SLD实现高空间分辨率OCT。
