Department of Food Science and Biotechnology, CHA University, Seongnam, Gyeonggi-do, South Korea.
IMMUNOBIOTECH Corp., Seoul, South Korea.
J Med Food. 2023 Sep;26(9):672-682. doi: 10.1089/jmf.2023.K.0066. Epub 2023 Jul 26.
This study researched the mineral composition of Korean washed-dehydrated solar salt (WDS) without bittern. It also evaluated the anticancer effects of doenjang (WDSD) prepared using WDS on azoxymethane (AOM)/dextran sodium sulfate (DSS)-induced colon cancer in C57BL/6 mice. The mineral composition of WDS showed lower Mg (11.71 ± 1.89 g/kg) and S (9.77 ± 2.88 g/kg) contents, and it was confirmed that mice in the WDSD group (AOM/DSS+WDSD) displayed significantly lower weight loss, colon length reduction, and tumor formation compared with the control (Con) group. In addition, pathologically, it was confirmed that the extent of epithelial cell damage and inflammation in the colon tissue of the WDSD group was restored to a state similar to that of the Nor group. Besides, WDSD regulated the protein expression of apoptosis (Bcl-2-associated X protein [Bax], B cell lymphoma-2 [Bcl-2], B cell lymphoma-extra large [Bcl-xL], and caspase 9, caspase 3), and p53, p21, and proinflammatory cytokines (interleukin [IL]-6, tumor necrosis factor [TNF]-), thereby inducing the apoptosis and cell cycle arrest of cancer cells and suppressing inflammation. In addition, the intestinal microbiota of the mice treated with WDSD were more diverse, with an abundance of , a lactic acid bacterium beneficial to colon health, was also a greater presence of , which showed antitumor effects. These results indicate that solar salts and their different processing methods affect their functional health-promoting properties. In addition, the inhibitory effect on colon cancer was further enhanced when doenjang was prepared with WDS with low Mg and S content.
本研究对无卤的韩国水洗脱水日晒盐（WDS）的矿物质组成进行了研究，并评估了使用 WDS 制备的味噌（WDSD）对 AOM/DSS 诱导的 C57BL/6 小鼠结肠癌的抗癌作用。WDS 的矿物质组成显示出较低的 Mg（11.71±1.89g/kg）和 S（9.77±2.88g/kg）含量，并且证实 WDSD 组（AOM/DSS+WDSD）的小鼠与对照组（Con）相比，体重减轻、结肠长度缩短和肿瘤形成明显减少。此外，病理上证实，WDSD 组结肠组织上皮细胞损伤和炎症的程度恢复到类似于 Nor 组的状态。此外，WDSD 调节了细胞凋亡（Bcl-2 相关 X 蛋白[Bax]、B 细胞淋巴瘤-2[Bcl-2]、B 细胞淋巴瘤-extra large[Bcl-xL]和半胱天冬酶 9、半胱天冬酶 3）和 p53、p21 和促炎细胞因子（白细胞介素[IL]-6、肿瘤坏死因子[TNF]-）的蛋白表达，从而诱导癌细胞凋亡和细胞周期停滞，抑制炎症。此外，用 WDSD 处理的小鼠的肠道微生物群更加多样化，有益结肠健康的乳酸菌的丰度也更高，而具有抗肿瘤作用的也更多。这些结果表明，日晒盐及其不同加工方法会影响其功能性健康促进特性。此外，当用低 Mg 和 S 含量的 WDS 制备味噌时，对结肠癌的抑制作用进一步增强。
